Activity-related calcium dynamics in motoneurons of the nucleus hypoglossus from mouse.

Abstract:

:A quantitative analysis of activity-related calcium dynamics was performed in motoneurons of the nucleus hypoglossus in the brain stem slice preparation from mouse by simultaneous patch-clamp and microfluorometric calcium measurements. Motoneurons were analyzed under in vitro conditions that kept them in a functionally intact state represented by rhythmic, inspiratory-related bursts of excitatory postsynaptic currents and associated action potential discharges. Bursts of electrical activity were paralleled by somatic calcium transients resulting from calcium influx through voltage-activated calcium channels, where each action potential accounted for a calcium-mediated charge influx around 2 pC into the somatic compartment. Under in vivo conditions, rhythmic-respiratory activity in young mice occurred at frequencies up to 5 Hz, demonstrating the necessity for rapid calcium elevation and recovery in respiratory-related neurons. The quantitative analysis of hypoglossal calcium homeostasis identified an average extrusion rate, but an exceptionally low endogenous calcium binding capacity as cellular parameters accounting for rapid calcium signaling. Our results suggest that dynamics of somatic calcium transients 1) define an upper limit for the maximum frequency of respiratory-related burst discharges and 2) represent a potentially dangerous determinant of intracellular calcium profiles during pathophysiological and/or excitotoxic conditions.

journal_name

J Neurophysiol

authors

Lips MB,Keller BU

doi

10.1152/jn.1999.82.6.2936

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

2936-46

issue

6

eissn

0022-3077

issn

1522-1598

journal_volume

82

pub_type

杂志文章
  • Cellular mechanisms underlying intrathalamic augmenting responses of reticular and relay neurons.

    abstract::Augmenting (or incremental) responses are progressively growing potentials elicited by 5- to 15-Hz stimulation within the thalamus, cerebral cortex, or by setting into action reciprocal thalamocortical neuronal loops. These responses are associated with short-term plasticity processes in thalamic and cortical neurons....

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.79.5.2716

    authors: Timofeev I,Steriade M

    更新日期:1998-05-01 00:00:00

  • Existence in the nucleus incertus of the cat of horizontal-eye-movement-related neurons projecting to the cerebellar flocculus.

    abstract::1. Properties of nucleus incertus (NIC) neurons projecting to the cerebellar flocculus were studied in alert cats by using chronic unit and eye movement recording and antidromic activation. Projection of these neurons onto the flocculus was verified with retrograde transport of horseradish peroxidase after injections ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1995.74.3.1367

    authors: Cheron G,Saussez S,Gerrits N,Godaux E

    更新日期:1995-09-01 00:00:00

  • Modulation of sympathetic and somatomotor function by the ventromedial medulla.

    abstract::The ventromedial medulla is implicated in a variety of functions including nociceptive and cardiovascular modulation and the control of thermoregulation. To determine whether single microinjections into the ventromedial medulla elicit changes in one or multiple functional systems, the GABA(A) receptor antagonist bicuc...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00089.2004

    authors: Nason MW Jr,Mason P

    更新日期:2004-07-01 00:00:00

  • Mixtures of octopamine and serotonin have nonadditive effects on the CNS of the medicinal leech.

    abstract::It is well established that neural networks respond to a wide variety of modulatory substances by which they can become reconfigured, yet few studies have examined the effects of neurotransmitter mixtures on such networks. In a previous study of the medicinal leech using triple intracellular recordings, we found that ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2001.85.5.2039

    authors: Mesce KA,Crisp KM,Gilchrist LS

    更新日期:2001-05-01 00:00:00

  • Chemosensory burst coding by mouse vomeronasal sensory neurons.

    abstract::The capabilities of any sensory system are ultimately constrained by the properties of the sensory neurons: the ability to detect and represent stimuli is limited by noise due to spontaneous activity, and optimal decoding in downstream circuitry must be matched to the nature of the encoding performed at the input. Her...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00108.2011

    authors: Arnson HA,Holy TE

    更新日期:2011-07-01 00:00:00

  • Analogous responses in the nucleus accumbens and cingulate cortex to pain onset (aversion) and offset (relief) in rats and humans.

    abstract::In humans, functional magnetic resonance imaging (fMRI) activity in the anterior cingulate cortex (ACC) and the nucleus accumbens (NAc) appears to reflect affective and motivational aspects of pain. The responses of this reward-aversion circuit to relief of pain, however, have not been investigated in detail. Moreover...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00284.2013

    authors: Becerra L,Navratilova E,Porreca F,Borsook D

    更新日期:2013-09-01 00:00:00

  • Dimethyl disulfide exerts insecticidal neurotoxicity through mitochondrial dysfunction and activation of insect K(ATP) channels.

    abstract::The plant-derived insecticides have introduced a new concept in insecticide research. In response to insect attacks, some plants can release volatile sulfur compounds such as dimethyl disulfide (DMDS) in the atmosphere, which are lethal for the generalist insects. We demonstrate that DMDS induced an uncommon complex n...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01096.2002

    authors: Dugravot S,Grolleau F,Macherel D,Rochetaing A,Hue B,Stankiewicz M,Huignard J,Lapied B

    更新日期:2003-07-01 00:00:00

  • Central input to primary afferent neurons in crayfish, Pacifastacus leniusculus, is correlated with rhythmic motor output of thoracic ganglia.

    abstract::A preparation is described in which the thoracic ganglia of the crayfish are isolated together with the thoracocoxal muscle receptor organ (TCMRO) of the fourth leg. This preparation allows intracellular analysis of both centrally generated and reflex activity in leg motor neurons (MNs). The isolated thoracic ganglia ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1986.55.4.678

    authors: Sillar KT,Skorupski P

    更新日期:1986-04-01 00:00:00

  • Comparison of morphine and kainic acid microinjections into identical PAG sites on the activity of RVM neurons.

    abstract::The rostral ventromedial medulla (RVM) modulates nociception through changes in the activity of two classes of neuron, ON- and OFF-cells. The activity of these neurons is regulated, in part, by input from the periaqueductal gray (PAG). The objective of this study was to determine whether PAG-mediated antinociception i...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2002.88.4.1707

    authors: Tortorici V,Morgan MM

    更新日期:2002-10-01 00:00:00

  • Tactile detection of slip: surface microgeometry and peripheral neural codes.

    abstract::1. The role of the microgeometry of planar surfaces in the detection of sliding of the surfaces on human and monkey fingerpads was investigated. By the use of a servo-controlled tactile stimulator to press and stroke glass plates on passive fingerpads of human subjects, the ability of humans to discriminate the direct...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1990.63.6.1323

    authors: Srinivasan MA,Whitehouse JM,LaMotte RH

    更新日期:1990-06-01 00:00:00

  • Microstructural organizational patterns in the human corticostriatal system.

    abstract::The axons that project into the striatum are known to segregate according to macroscopic cortical systems; however, the within-region organization of these fibers has yet to be described in humans. We used in vivo fiber tractography, in neurologically healthy adults, to map white matter bundles that originate in diffe...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00995.2011

    authors: Verstynen TD,Badre D,Jarbo K,Schneider W

    更新日期:2012-06-01 00:00:00

  • Persistent synaptic activity produces long-lasting enhancement of endocannabinoid modulation and alters long-term synaptic plasticity.

    abstract::Learning and memory are thought to involve activity-dependent changes in synaptic efficacy such as long-term potentiation (LTP) and long-term depression (LTD). Recent studies have indicated that endocannabinoid-dependent modulation of inhibitory transmission facilitates induction of hippocampal LTP and that endocannab...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01228.2006

    authors: Zhu PJ,Lovinger DM

    更新日期:2007-06-01 00:00:00

  • Single-unit responses to natural vestibular stimuli and eye movements in deep cerebellar nuclei of the alert rhesus monkey.

    abstract::To study the possible role of the cerebellum in the vestibular-ocular reflex, extracellular responses of cerebellar nuclear neurons were recorded in awake monkeys during natural vestibular stimulation; 115 neurons in the fastigial nucleus responded to horizontal sinusoidal accelerations applied to the head by means of...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1975.38.3.627

    authors: Gardner EP,Fuchs AF

    更新日期:1975-05-01 00:00:00

  • The development of transmission at an identified molluscan synapse. II. A quantal analysis of transmission.

    abstract::1. A quantal analysis of transmission at the identified molluscan synapse, V2-RPr1, was performed during development. The study was intended to determine the pre- and postsynaptic contributions to the marked changes in transmitter release described in the previous report. 2. The success of the quantal analysis was pre...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1988.60.6.2211

    authors: Pawson PA,Chase R

    更新日期:1988-12-01 00:00:00

  • The suppressive influence of moving textured backgrounds on responses of cat striate neurons to moving bars.

    abstract::The suppressive action of a moving textured background on responses to moving bars was investigated in 118 striate neurons, 19 dorsal lateral geniculate neurons, and 5 perigeniculate neurons in paralyzed and anesthetized cats. In standard conditions the background was a two-dimensional (2D) noise pattern, the bar move...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1987.57.6.1767

    authors: Gulyás B,Orban GA,Duysens J,Maes H

    更新日期:1987-06-01 00:00:00

  • Selection of head and whisker coordination strategies during goal-oriented active touch.

    abstract::In the rodent whisker system, a key model for neural processing and behavioral choices during active sensing, whisker motion is increasingly recognized as only part of a broader motor repertoire employed by rodents during active touch. In particular, recent studies suggest whisker and head motions are tightly coordina...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00465.2015

    authors: Schroeder JB,Ritt JT

    更新日期:2016-04-01 00:00:00

  • Glutamate currents in morphologically identified human dentate granule cells in temporal lobe epilepsy.

    abstract::Glutamate-receptor-mediated synaptic transmission was studied in morphologically identified hippocampal dentate granule cells (DGCs; n = 31) with the use of whole cell patch-clamp recording and intracellular injection of biocytin or Lucifer yellow in slices prepared from surgically removed medial temporal lobe specime...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.77.6.3355

    authors: Isokawa M,Levesque M,Fried I,Engel J Jr

    更新日期:1997-06-01 00:00:00

  • Sudden drop in ground support produces force-related unload response in human overground walking.

    abstract::Humans maneuver easily over uneven terrain. To maintain smooth and efficient gait the motor system needs to adapt the locomotor output to the walking environment. In the present study we investigate the role of sensory feedback in adjusting the soleus muscle activity during overground walking in 19 healthy volunteers....

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.91175.2008

    authors: Af Klint R,Nielsen JB,Sinkjaer T,Grey MJ

    更新日期:2009-04-01 00:00:00

  • Maintaining grip: anticipatory and reactive EEG responses to load perturbations.

    abstract::Previous behavioral work has shown the existence of both anticipatory and reactive grip force responses to predictable load perturbations, but how the brain implements anticipatory control remains unclear. Here we recorded electroencephalographs while participants were subjected to predictable and unpredictable extern...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01112.2006

    authors: Kourtis D,Kwok HF,Roach N,Wing AM,Praamstra P

    更新日期:2008-02-01 00:00:00

  • Binary mapping of cortical spike trains in short-term memory.

    abstract::Microelectrode studies in monkeys performing short-term memory tasks show the sustained elevated discharge of cortical neurons during the retention of recalled sensory information. Cortical cells that are part of memory networks are assumed to receive numerous inputs of excitatory as well as inhibitory nature and loca...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.77.4.2219

    authors: Bodner M,Zhou YD,Fuster JM

    更新日期:1997-04-01 00:00:00

  • Decreased heart rate and enhanced sinus arrhythmia during interictal sleep demonstrate autonomic imbalance in generalized epilepsy.

    abstract::We hypothesized that epilepsy affects the activity of the autonomic nervous system even in the absence of seizures, which should manifest as differences in heart rate variability (HRV) and cardiac cycle. To test this hypothesis, we investigated ECG traces of 91 children and adolescents with generalized epilepsy and 25...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01120.2015

    authors: Sivakumar SS,Namath AG,Tuxhorn IE,Lewis SJ,Galán RF

    更新日期:2016-04-01 00:00:00

  • Covert orienting of attention in macaques. I. Effects of behavioral context.

    abstract::1. A task was used by Posner (1980) to measure shifts of attention that occurred covertly, in the absence of an eye movement or other orienting response. This paradigm was used here to assess the nature of covert attentional orienting in monkeys to develop an animal model for neurophysiological studies. Shifts of atte...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1993.70.1.431

    authors: Bowman EM,Brown VJ,Kertzman C,Schwarz U,Robinson DL

    更新日期:1993-07-01 00:00:00

  • Cooperation of muscle and cutaneous afferents in the feedback of contraction to peroneal motoneurons.

    abstract::Peroneal motoneurons were recorded intracellularly in anesthetized cats during sustained submaximal contractions of peroneus brevis muscle (PB) elicited by repetitive electrical stimulation of motor axons in the distal portion of cut ventral root filaments. Mechanical stimulation of the territory innervated by the sup...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.83.6.3201

    authors: Perrier JF,Lamotte D'Incamps B,Kouchtir-Devanne N,Jami L,Zytnicki D

    更新日期:2000-06-01 00:00:00

  • Learning and generalization in an isometric visuomotor task.

    abstract::Adaptation is a prominent feature of the human motor system and has been studied extensively in reaching movements. This study characterizes adaptation and generalization during isometric reaching in which the arm remains stationary and the participant controls a virtual cursor via force applied by the hand. We measur...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00255.2014

    authors: Rotella MF,Nisky I,Koehler M,Rinderknecht MD,Bastian AJ,Okamura AM

    更新日期:2015-03-15 00:00:00

  • Decomposition of surface EMG signals from cyclic dynamic contractions.

    abstract::Over the past 3 decades, various algorithms used to decompose the electromyographic (EMG) signal into its constituent motor unit action potentials (MUAPs) have been reported. All are limited to decomposing EMG signals from isometric contraction. In this report, we describe a successful approach to decomposing the surf...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00555.2014

    authors: De Luca CJ,Chang SS,Roy SH,Kline JC,Nawab SH

    更新日期:2015-03-15 00:00:00

  • Sleep-like states modulate functional connectivity in the rat olfactory system.

    abstract::The present study was an examination of state-dependent functional connectivity during spontaneous activity between the piriform cortex and its upstream and downstream connections. Rats were anesthetized with urethan and allowed to spontaneously cycle between fast- and slow-wave states similar to fast- and slow-wave s...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00711.2010

    authors: Wilson DA,Yan X

    更新日期:2010-12-01 00:00:00

  • Interjoint coordination in the stick insect leg-control system: the role of positional signaling.

    abstract::Interjoint coordination is essential for proper walking behavior in multi-jointed insect legs. We have shown previously that movement signals from the femur-tibia (FT) joint can shape motor activity of the adjacent coxa-trochanter (CT) joint in the stick insect, Carausius morosus. Here, we present data on the role of ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00637.2002

    authors: Bucher D,Akay T,DiCaprio RA,Buschges A

    更新日期:2003-03-01 00:00:00

  • Neuronal responsiveness in the ventrobasal thalamic complex of rats with an experimental peripheral mononeuropathy.

    abstract::1. Single-unit recordings were made, under moderate gaseous anesthesia (33% O2-66% N2O + 0.5/0.6% halothane), in the ventrobasal (VB) thalamic complex of rats (n = 42) with a mononeuropathy created 2-3 wk beforehand, by four loose ligatures around the common sciatic nerve. Before the recording session, three behaviora...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1990.64.5.1537

    authors: Guilbaud G,Benoist JM,Jazat F,Gautron M

    更新日期:1990-11-01 00:00:00

  • Strength-duration relationship for extracellular neural stimulation: numerical and analytical models.

    abstract::The strength-duration relationship for extracellular stimulation is often assumed to be similar to the classical intracellular stimulation model, with a slope asymptotically approaching 1/τ at pulse durations shorter than chronaxy. We modeled extracellular neural stimulation numerically and analytically for several ce...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00343.2010

    authors: Boinagrov D,Loudin J,Palanker D

    更新日期:2010-10-01 00:00:00

  • Antisaccade cost is modulated by contextual experience of location probability.

    abstract::It is well known that pro- and antisaccades may deploy different cognitive processes. However, the specific reason why antisaccades have longer latencies than prosaccades is still under debate. In three experiments, we studied the factors contributing to the antisaccade cost by taking attentional orienting and target ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00815.2009

    authors: Liu CL,Chiau HY,Tseng P,Hung DL,Tzeng OJ,Muggleton NG,Juan CH

    更新日期:2010-03-01 00:00:00