Abstract:
:A quantitative analysis of activity-related calcium dynamics was performed in motoneurons of the nucleus hypoglossus in the brain stem slice preparation from mouse by simultaneous patch-clamp and microfluorometric calcium measurements. Motoneurons were analyzed under in vitro conditions that kept them in a functionally intact state represented by rhythmic, inspiratory-related bursts of excitatory postsynaptic currents and associated action potential discharges. Bursts of electrical activity were paralleled by somatic calcium transients resulting from calcium influx through voltage-activated calcium channels, where each action potential accounted for a calcium-mediated charge influx around 2 pC into the somatic compartment. Under in vivo conditions, rhythmic-respiratory activity in young mice occurred at frequencies up to 5 Hz, demonstrating the necessity for rapid calcium elevation and recovery in respiratory-related neurons. The quantitative analysis of hypoglossal calcium homeostasis identified an average extrusion rate, but an exceptionally low endogenous calcium binding capacity as cellular parameters accounting for rapid calcium signaling. Our results suggest that dynamics of somatic calcium transients 1) define an upper limit for the maximum frequency of respiratory-related burst discharges and 2) represent a potentially dangerous determinant of intracellular calcium profiles during pathophysiological and/or excitotoxic conditions.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Lips MB,Keller BUdoi
10.1152/jn.1999.82.6.2936subject
Has Abstractpub_date
1999-12-01 00:00:00pages
2936-46issue
6eissn
0022-3077issn
1522-1598journal_volume
82pub_type
杂志文章abstract::Augmenting (or incremental) responses are progressively growing potentials elicited by 5- to 15-Hz stimulation within the thalamus, cerebral cortex, or by setting into action reciprocal thalamocortical neuronal loops. These responses are associated with short-term plasticity processes in thalamic and cortical neurons....
journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01228.2006
更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-05-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1988.60.6.2211
更新日期:1988-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.6.3355
更新日期:1997-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.91175.2008
更新日期:2009-04-01 00:00:00
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journal_title:Journal of neurophysiology
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更新日期:2008-02-01 00:00:00
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journal_title:Journal of neurophysiology
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journal_title:Journal of neurophysiology
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更新日期:2000-06-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2015-03-15 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2015-03-15 00:00:00
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journal_title:Journal of neurophysiology
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更新日期:2010-12-01 00:00:00
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journal_title:Journal of neurophysiology
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00