Abstract:
:Mammalian cell growth is governed by regulatory activities that include the products of genes such as c-myc and ras that act early in G1, as well as the E2F family of transcription factors that accumulate later in G1 to regulate the expression of genes involved in DNA replication. Previous work has shown that the expression of the E2F1, E2F2, and E2F3 gene products is tightly regulated by cell growth. To further explore the mechanisms controlling accumulation of E2F activity, we have isolated genomic sequences flanking the 5' region of the E2F2 coding sequence. Various assays demonstrate promoter activity in this sequence that reproduces the normal control of E2F2 expression during a growth stimulation. Sequence comparison reveals the presence of a variety of known transcription factor binding sites, including E-box elements that are consensus Myc binding sites, as well as E2F binding sites. We demonstrate that the E-box elements, which we show can function as Myc-responsive sites, contribute in a positive fashion to promoter function. We also find that E2F-dependent negative regulation in quiescent cells plays a significant role in the cell growth-dependent control of the promoter, similar to the regulation of the E2F1 gene promoter.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Sears R,Ohtani K,Nevins JRdoi
10.1128/mcb.17.9.5227subject
Has Abstractpub_date
1997-09-01 00:00:00pages
5227-35issue
9eissn
0270-7306issn
1098-5549journal_volume
17pub_type
杂志文章abstract::The Ras pathway is critical for the development and function of T lymphocytes. The stimulation of this GTPase in T cells occurs primarily through the Vav1- and phospholipase C-gamma1-dependent activation of RasGRP1, a diacylglycerol-responsive Ras GDP/GTP exchange factor. Here, we show that a second exchange factor, R...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00912-07
更新日期:2007-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/MCB.00205-14
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pub_type: 杂志文章
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更新日期:1986-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.24.3.1378-1386.2004
更新日期:2004-02-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00157-08
更新日期:2008-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/MCB.00544-07
更新日期:2007-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.20.23.8903-8915.2000
更新日期:2000-12-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.7.5.1917
更新日期:1987-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.5.5.1170
更新日期:1985-05-01 00:00:00