Abstract:
:Granzyme B (GrB) is the primary molecular mediator of apoptosis by cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is a unique mammalian aspartic acid-cleaving serine protease. On T cell receptor activation, GrB is released from the CTL cytoplasmic granules by exocytosis, enters the target cells and, in the presence of the granule pore-forming protein perforin, it initiates the processing of caspases and apoptosis. GrB apoptosis is also activated by adenovirus, which can effectively replace perforin. Methods for the purification and quantitation of GrB and perforin, and the preparation and titration of adenovirus, are described. In addition, methods for application of these reagents to the initiation of apoptosis in tumor target cells, with several assays for detecting GrB apoptotic activity, are detailed.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Shi L,Yang X,Froelich CJ,Greenberg AHdoi
10.1016/s0076-6879(00)22013-2subject
Has Abstractpub_date
2000-01-01 00:00:00pages
125-43eissn
0076-6879issn
1557-7988pii
S0076-6879(00)22013-2journal_volume
322pub_type
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