Abstract：
:The methods described here concern the use specialized glassware to achieve accurate sub-micromolar to low-millimolar dioxygen concentrations specifically for the study of flavoproteins. Since the early 1960s, these methods have been passed through the lineage of flavin enzymologists almost exclusively as an oral tradition. Given that these methods have been used to systematically reveal a multitude of natural phenomena, it is a curiosity that they have not been comprehensively committed to print prior to this entry. It should be noted that the author is describing these methods with scant added modernity and should not be attributed with their invention. The investigators to whom such ingenuity should be accredited include David P. Ballou, Quentin H. Gibson, Vincent Massey, Charles H. Williams, and Graham Palmer (Foust, Burleigh, & Mayhew, 1969; Gibson, Massey, & Atherton, 1962; Massey, Brumby, Komai, & Palmer, 1969; Massey, Gibson, & Veeger, 1960), who, out of necessity, developed the vessel design and approach over a period of two decades.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Moran GRdoi
10.1016/bs.mie.2019.03.005subject
Has Abstractpub_date
2019-01-01 00:00:00pages
27-49eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30086-2journal_volume
620pub_type
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journal_title：Methods in enzymology
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journal_title：Methods in enzymology
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journal_title：Methods in enzymology
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更新日期：2018-01-01 00:00:00
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journal_title：Methods in enzymology
pub_type： 杂志文章
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journal_title：Methods in enzymology
pub_type： 杂志文章
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