Abstract:
:Nitrite and nitrate represent the final products of nitric oxide (NO) oxidation pathways, and their hematic concentrations are frequently assessed as an index of systemic NO production. However, their intake with food can influence their levels. Nitrite and nitrate could have a role by producing NO, because nitrite can release NO after reaction with deoxyhemoglobin and dietary nitrate can be reduced substantially to nitrite by commensal bacteria in the oral cavity. Different methods have been applied for nitrite/nitrate detection, with the most commonly used being the spectrophotometric assay based on the Griess reagent. However, a reference methodology for these determinations is still missing and many possible interferences have been reported. This chapter assesses how different experimental conditions can influence the results when detecting nitrite and nitrate in human plasma by the Griess assay and provides a simple method characterized by high reproducibility and minimized interferences by plasma constituents.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Giustarini D,Rossi R,Milzani A,Dalle-Donne Idoi
10.1016/S0076-6879(07)00823-3subject
Has Abstractpub_date
2008-01-01 00:00:00pages
361-80eissn
0076-6879issn
1557-7988pii
S0076-6879(07)00823-3journal_volume
440pub_type
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