Abstract:
:Mitochondrial RNAs in the myxomycete Physarum polycephalum differ from the templates from which they are transcribed in defined ways. Most transcripts contain nucleotides that are not present in their respective genes. These "extra" nucleotides are added during RNA synthesis by an unknown mechanism. Other differences observed between Physarum mitochondrial RNAs and the mitochondrial genome include nucleotide deletions, C to U changes, and the replacement of one nucleotide for another at the 5' end of tRNAs. All of these alterations are remarkably precise and highly efficient in vivo. Many of these editing events can be replicated in vitro, and here we describe both the in vitro systems used to study editing in Physarum mitochondria and the assays that have been developed to assess the extent of editing of RNAs generated in these systems at individual sites.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Byrne EM,Visomirski-Robic L,Cheng YW,Rhee AC,Gott JMdoi
10.1016/S0076-6879(07)24007-8subject
Has Abstractpub_date
2007-01-01 00:00:00pages
143-72eissn
0076-6879issn
1557-7988pii
S0076-6879(07)24007-8journal_volume
424pub_type
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