Abstract：
:In polarized epithelial cells, the tight junction has been ascribed several functions including the regulation of the paracellular permeability, an impediment to the diffusion of molecules between the apical and basolateral domains, a site of delivery of transport vesicles for basolateral proteins, and a scaffold for structural and signaling molecules. The tight junction is anchored physically into the apical actin cytoskeleton circumscribing the cell, which is known as the perijunctional actomyosin ring. This connection was first suggested by experiments using the actin depolymerizing drug cytochalasin, which was also found to disrupt the transepithelial permeability. Since then a large number of studies have reported the effects of drugs, molecular tools, or physiological and pathological conditions that alter coordinately actin organization and the tight junction. In support of this model, proteins of the tight junction, such as the members of the ZO family and occludin, have been shown to bind to actin. However, very little is known regarding the molecular mechanisms by which the actin cytoskeleton modulates tight junction functions. We have studied the role of the Exchange Factor for Arf6, EFA6, in tight junction assembly. By combining a large panel of methods, including morphological, physiological, and biochemical, described in detail hereafter we demonstrated that EFA6 plays a role in the physical association of the tight junction to the perijunctional actomyosin ring.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Luton Fdoi
10.1016/S0076-6879(05)04029-2subject
Has Abstractpub_date
2005-01-01 00:00:00pages
332-45eissn
0076-6879issn
1557-7988pii
S0076-6879(05)04029-2journal_volume
404pub_type
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