Abstract:
:The bromodomain (BrD) represents an evolutionarily conserved protein domain whose function mostly is to recognize acetylated lysine residues in histones and nuclear proteins in regulation of gene transcription in chromatin. The highly conserved BrD structure features an unusual left-handed, antiparallel four-helix bundle and a hydrophobic pocket between the interhelical ZA and BC loops important for acetyl-lysine binding. Many proteins, particularly transcriptional activators, contain BrDs, and mutation or deletion of the BrDs impairs the protein function, implying their critical role in human biology and disease. In this chapter, we provide general protocols of the preparation, biochemical analysis, and structure determination of BrDs, aiming to offer a general guideline for structural and biochemical functional characterization of BrD-containing proteins.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Ren C,Zeng L,Zhou MMdoi
10.1016/bs.mie.2016.01.018subject
Has Abstractpub_date
2016-01-01 00:00:00pages
321-43eissn
0076-6879issn
1557-7988pii
S0076-6879(16)00063-Xjournal_volume
573pub_type
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