Abstract:
:Over the last 50 years cytochrome c has been used as a model system for studying electron transfer and protein folding processes. Recently, convincing evidence has been provided that this protein is also involved in other biological processes such as the apoptosis and α-synuclein aggregation. Numerous lines of evidence suggest that the diversity of the functional properties of cytochrome c is linked to its conformational plasticity. This chapter introduces circular dichroism and absorption spectroscopy, as an ideal tool to explore this protein's conformational in solution. Besides assisting in distinguishing different conformations and in obtaining the equilibrium thermodynamics of the transitions between them, the two spectroscopies can also be used to explore details of heme-protein interaction, for example, the influence of the external electric field on the prosthetic heme group.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Schweitzer-Stenner R,Hagarman A,Verbaro D,Soffer JBdoi
10.1016/S0076-6879(09)66006-7subject
Has Abstractpub_date
2009-01-01 00:00:00pages
109-53eissn
0076-6879issn
1557-7988pii
S0076-6879(09)66006-7journal_volume
466pub_type
杂志文章abstract::Antibodies are excellent binding proteins that have found numerous applications in biological research, biotechnology, and medicine. Characterization of their ligand binding properties has long been, and continues to be, the focus of many researchers. Antibodies are also perfect test systems which can be used for the ...
journal_title:Methods in enzymology
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abstract::Scanning fluorescence correlation spectroscopy (sFCS) is the generic term for a group of fluorescence correlation techniques where the measurement volume is moved across the sample in a defined way. The introduction of scanning is motivated by its ability to alleviate or remove several distinct problems often encounte...
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journal_title:Methods in enzymology
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abstract::Neutron reflectometry (NR) is uniquely positioned in structural biology, because of its ability to characterize biomolecular interfacial architectures like lipid membranes and membrane-associated proteins nondestructively and in their native environment. Mimicking biological processes, samples can be manipulated and t...
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2014.10.051
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abstract::High-content screening (HCS), a combination of fluorescence microscopic imaging and automated image analysis, has become a frequently applied tool to study test compound effects in cellular disease-modeling systems. This chapter describes the measurement of G protein-coupled receptor (GPCR) internalization in the HCS ...
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章,评审
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abstract::Nonsense-mediated mRNA decay (NMD) is activated by exon-junction complexes (EJCs) that are located downstream of the termination codon of the substrate mRNAs. This situation can be imitated by tethering components of the EJC to the 3' untranslated region (3' UTR) of a reporter mRNA. Here we describe the detailed use o...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(08)02623-2
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journal_title:Methods in enzymology
pub_type: 杂志文章
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/B978-0-12-391856-7.00039-1
更新日期:2012-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2019.03.005
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