Abstract:
:Glycosylation is a complex form of protein modification occurring in the secretory pathway. The addition of N- and O-glycans affects intracellular processes like the folding and trafficking of most glycoproteins. To better understand the impact of glycosylation in protein folding and maturation, parameters like glycosylation site occupancy and oligosaccharide structure must be measured quantitatively. In this chapter, we describe current methods enabling the determination of N-glycosylation by assessment of cellular dolichol phosphate levels, dolichol-linked oligosaccharides, and the occupancy of N-glycosylation sites. We also provide detailed methods for the analysis of O-glycosylation, whose role in intracellular protein maturation is often overlooked.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Hülsmeier AJ,Welti M,Hennet Tdoi
10.1016/B978-0-12-385928-0.00010-9subject
Has Abstractpub_date
2011-01-01 00:00:00pages
163-82eissn
0076-6879issn
1557-7988pii
B978-0-12-385928-0.00010-9journal_volume
491pub_type
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