Abstract:
:Advances in chemical synthesis and characterization of nucleic acids allows for atom-specific modification of complex RNAs, such as present in RNA editing substrates. By preparing substrates for ADARs by chemical synthesis, it is possible to subtly alter the structure of the edited nucleotide. Evaluating the effect these changes have on the rate of enzyme-catalyzed deamination reveals features of the editing reaction and guides the design of inhibitors. We describe the synthesis of select nucleoside analog phosphoramidites and their incorporation into RNAs that mimic known editing sites by solid phase synthesis, and analyze the interaction of these synthetic RNAs with ADARs using deamination kinetics and quantitative gel mobility shift assays.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Maydanovych O,Easterwood LM,Cui T,Véliz EA,Pokharel S,Beal PAdoi
10.1016/S0076-6879(07)24017-0subject
Has Abstractpub_date
2007-01-01 00:00:00pages
369-86eissn
0076-6879issn
1557-7988pii
S0076-6879(07)24017-0journal_volume
424pub_type
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