Abstract:
:Antibody unfolding and aggregation have been gaining increasing concern as the need for pharmaceutical antibodies with high quality has increased. Assays for continuous application of severe stress to antibodies, which are typically employed to evaluate tolerance to aggregation or to understand the mechanism of aggregation, induce both unfolding and aggregation simultaneously. In this chapter, we describe the pH-shift stress on antibodies that uses a low pH solution to unfold the antibody and a neutralizing solution to induce folding and the aggregation. This pH-shift technique with light scattering analysis will be a useful assay to evaluate antibody aggregation at a neutral pH and ambient temperature without generation of unfolded molecules during aggregation.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Imamura H,Honda Sdoi
10.1016/bs.mie.2019.02.021subject
Has Abstractpub_date
2019-01-01 00:00:00pages
329-345eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30040-0journal_volume
622pub_type
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