Abstract:
:Synthetic hydrogen sulfide (H2S) donors are useful research tools as well as potential therapeutic agents. In this chapter, we report the detailed protocols for the synthesis and evaluation of a series of phosphorodithioate-based H2S donors. Fluorescence assays were used to determine H2S release from the donors in both aqueous buffers and in cultured cells. These donors were found to be slow-release donors, much like the well-known GYY4137. These donors also showed some protective effects against hydrogen peroxide (H2O2)-induced oxidative damage in myocytes.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Park CM,Xian Mdoi
10.1016/bs.mie.2014.11.032subject
Has Abstractpub_date
2015-01-01 00:00:00pages
127-42eissn
0076-6879issn
1557-7988pii
S0076-6879(14)00097-4journal_volume
554pub_type
杂志文章abstract::The properties of a collection of transposon Tn5 derivatives that generate reporter gene fusions and internal protein tags are summarized. Procedures utilizing several of the transposons for generating genes activated by Cre-loxP recombination and for creating large sequence-defined mutant libraries are described in d...
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journal_title:Methods in enzymology
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