Abstract:
:This protocol describes a general protein-to-protein cross-linking procedure using the water-soluble amine-reactive homobifunctional BS(3) (bis[sulfosuccinimidyl] suberate); however, the protocol can be easily adapted using other cross-linkers of similar properties. BS(3) is composed of two sulfo-NHS ester groups and an 11.4 Å linker. Sulfo-NHS ester groups react with primary amines in slightly alkaline conditions (pH 7.2-8.5) and yield stable amide bonds. The reaction releases N-hydroxysuccinimide (see an application of NHS esters on Labeling a protein with fluorophores using NHS ester derivitization).
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Alegria-Schaffer Adoi
10.1016/B978-0-12-420120-0.00006-2subject
Has Abstractpub_date
2014-01-01 00:00:00pages
81-7eissn
0076-6879issn
1557-7988pii
B978-0-12-420120-0.00006-2journal_volume
539pub_type
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