Abstract:
:Modular calcium-dependent carbohydrate-recognition domains (CRDs) of mammalian glycan-binding receptors (C-type lectins), engineered to have novel glycan-binding selectivity, have been developed as tools for the study of glycans on cell surfaces. Structure-based specificity swapping between domains can be complemented by empirical characterization of ligand-binding specificity using glycan arrays. Both natural and modified CRDs can be used as probes for detecting and isolating glycoproteins that bear specific glycan epitopes and that act as target ligands for glycan-binding receptors. CRD-based affinity chromatography facilitates proteomic and glycomic analysis of such ligands.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Powlesland AS,Quintero-Martinez A,Lim PG,Pipirou Z,Taylor ME,Drickamer Kdoi
10.1016/S0076-6879(10)80009-6subject
Has Abstractpub_date
2010-01-01 00:00:00pages
165-79eissn
0076-6879issn
1557-7988pii
S0076-6879(10)80009-6journal_volume
480pub_type
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