Abstract:
:As for other GPCRs, the oligomerization of glycoprotein hormone receptors (GPHRs) appears as critical event for receptor function. By means of modern techniques based on the BRET or FRET principle, GPHR oligomerization has been reported to explain several physiological and pathological conditions. In particular, the presence of oligomers was demonstrated not only in in vitro heterologous systems but also in in vivo tissues, and GPHR homodimerization appears associated with strong negative cooperativity, thus suggesting that one hormone molecule may be sufficient for receptor dimer stimulation. In addition, oligomerization has been reported to occur early during the posttranslational maturation process and to be involved in the dominant negative effect exerted by loss-of-function TSH receptor (TSHR) mutants, that are prevalently retained inside the cell, on the surface expression of wild-type receptors. This molecular mechanism thus explains the dominant inheritance of certain forms of TSH resistance. Here, we provide the description of the methods used in the original BRET, FRET, and HTRF-RET experiments.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Bonomi M,Persani Ldoi
10.1016/B978-0-12-391862-8.00020-Xsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
367-83eissn
0076-6879issn
1557-7988pii
B978-0-12-391862-8.00020-Xjournal_volume
521pub_type
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