Abstract:
:Cellular signaling and regulatory cascades often rely on post-translational modification of proteins, particularly phosphorylation, to quickly and effectively relay signals from a variety of inputs. Numerous kinases, the effectors of phosphorylation, and kinase networks have been implicated in human diseases. Until recently, an inability to produce high yields of physiologically phosphorylated proteins has proven to be a substantial barrier toward our understanding of many enzymatic processes. Orthogonal translation systems provide the means to overcome many of these limitations by enabling site-specific incorporation of phosphorylated amino acids into recombinantly expressed proteins. Site-by-site, combinatorial assessment of phosphorylation site function is unique to orthogonal translation system based approaches and offers unmatched precision in the study of PTM-enzymology, extending well beyond the scope of kinase biology.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Mohler K,Rinehart Jdoi
10.1016/bs.mie.2019.07.017subject
Has Abstractpub_date
2019-01-01 00:00:00pages
539-559eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30297-6journal_volume
626pub_type
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