Abstract:
:We have described a method using polyvalent peptide display on filamentous phage that can be used to identify ligands that bind to G protein beta gamma subunits. Also described is how to construct phage that have known beta gamma binding sequences fused to the coat protein to allow a competition analysis to be performed. Once selected or constructed, these phage-bearing beta gamma-binding peptides are powerful tools for mapping interaction sites for beta gamma binding proteins and can be used to begin to dissect the unique modes of binding for individual beta gamma subunit-regulated effectors.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Smrcka AV,Scott JKdoi
10.1016/s0076-6879(02)44740-4subject
Has Abstractpub_date
2002-01-01 00:00:00pages
557-76eissn
0076-6879issn
1557-7988journal_volume
344pub_type
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