Abstract:
:Inositol phosphates (InsPs) are an important group of eukaryotic messengers and mediate a wide range of processes. To elucidate the biological functions of these molecules, robust techniques to characterize inositol phosphate metabolism at the cellular level are highly sought after. This chapter provides a detailed protocol for the preparation of 13C-labeled myo-inositol, its use for metabolic labeling of mammalian and yeast cells, and the quantitative analysis of intracellular InsP pools from cell extracts using NMR spectroscopy.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Puschmann R,Harmel RK,Fiedler Ddoi
10.1016/bs.mie.2020.04.035subject
Has Abstractpub_date
2020-01-01 00:00:00pages
35-52eissn
0076-6879issn
1557-7988pii
S0076-6879(20)30163-4journal_volume
641pub_type
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