Abstract:
:In this chapter we discuss methods to study autophagic cell death. A large body of evidence demonstrates that autophagy is a cell survival mechanism in response to starvation. The role of autophagy in cell death, however, has long been controversial. Recently, molecular approaches have provided direct evidence that autophagy contributes to cell death in certain contexts. We begin this chapter by outlining methods to quantify cell death, for example by assaying for cell viability. Next, we discuss methods to measure processes involved in cell death, such as caspase activation and autophagy. Finally, we discuss methods to genetically or chemically perturb autophagy to test whether autophagy is required for cell death. Together, these approaches provide a guide to investigate the relationship between autophagy and cell death.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Lenardo MJ,McPhee CK,Yu Ldoi
10.1016/S0076-6879(08)04002-0subject
Has Abstractpub_date
2009-01-01 00:00:00pages
17-31eissn
0076-6879issn
1557-7988pii
S0076-6879(08)04002-0journal_volume
453pub_type
杂志文章abstract::Astrocytes are the most abundant cell type in the brain and are a crucial part of solving its mysteries. Originally assumed to be passive supporting cells, astrocyte's functions are now recognized to include active modulation and information processing at the neural synapse. The full extent of the astrocyte contributi...
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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