Abstract:
:Guanine nucleotide dissociation inhibitor (GDI) is a central regulator of Rab GTPase family members. GDI recycles Rab proteins from the membrane and sequesters the inactive GDP-bound form of Rab in the cytosol for use in multiple rounds of transport. The balance between the membrane-bound form of Rab and the cytosolic reserve pool of the Rab-GDI complex is critical for vesicular trafficking between membrane compartments. Recycling of Rab GTPases is likely to require a membrane-bound complex of GDI, Hsp90, and Rab given that alphaGDI-dependent recycling of Rab3A at the synapse and neurotransmitter transmitter release is inhibited by Hsp90-specific inhibitors. Here we describe methods required for establishing the dependence of Rab recycling pathways on Hsp90 in vitro.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Chen CY,Sakisaka T,Balch WEdoi
10.1016/S0076-6879(05)03029-6subject
Has Abstractpub_date
2005-01-01 00:00:00pages
339-47eissn
0076-6879issn
1557-7988pii
S0076-6879(05)03029-6journal_volume
403pub_type
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