Abstract:
:Respiration is widely used for evaluation of the metabolic capabilities or physiological state of the microbial culture. This chapter describes novel approaches for characterization of respiration at a single cell level: (1) flow cytometry-based redox sensing (FCRS) of actively metabolizing microbes; (2) respiration response imaging (RRI) for real-time detection of substrate stimulated redox responses of individual cells; (3) respiration detection system: microobservation chamber (RDS: MC), a single cell analysis system for carrying out the physiological and genomic profiling of cells capable of respiring C(1) compounds. The techniques are suitable for description of physiological heterogeneity among cells in a single microbial population and could be used to characterize distribution of methylotrophic ability among microbial cells in the natural environmental samples.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Konopka MC,McQuaide S,Ojala DS,Kalyuzhnaya MG,Lidstrom MEdoi
10.1016/B978-0-12-386905-0.00010-3subject
Has Abstractpub_date
2011-01-01 00:00:00pages
149-66eissn
0076-6879issn
1557-7988pii
B978-0-12-386905-0.00010-3journal_volume
495pub_type
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