Abstract:
:Technical innovations in structural probing have drastically advanced the field of RNA structure analysis. These advances have led to parallel approaches developed in separate labs for analyzing RNA structure and dynamics. With the wealth of methodologies available, it can be difficult to determine which is best suited for a given application. Here, using a long, highly structured viral RNA as an example (the positive strand genome of Hepatitis C Virus), we present a semi-comprehensive analysis and describe the major approaches for analyzing the architecture of RNA that is modified with structure-sensitive probes. Additionally, we present an updated method for generating in vitro transcribed and folded RNA that maintains native secondary structures in long RNA molecules. We anticipate that the methods described here will streamline the use of current approaches and help investigators who are unfamiliar with structure probing, obviating the need for time-consuming and expensive optimization.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Adams RL,Huston NC,Tavares RCA,Pyle AMdoi
10.1016/bs.mie.2019.04.002subject
Has Abstractpub_date
2019-01-01 00:00:00pages
249-289eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30117-Xjournal_volume
623pub_type
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