Abstract：
:Making error-free, custom DNA assemblies from potentially faulty building blocks is a fundamental challenge in synthetic biology. Here, we show how recursion can be used to address this challenge using a recursive procedure that constructs error-free DNA molecules and their libraries from error-prone synthetic oligonucleotides and naturally existing DNA. Specifically, we describe how divide and conquer (D&C), the quintessential recursive problem-solving technique, is applied in silico to divide target DNA sequences into overlapping, albeit error prone, oligonucleotides, and how recursive construction is applied in vitro to combine them to form error-prone DNA molecules. To correct DNA sequence errors, error-free fragments of these molecules are then identified, extracted, and used as new, typically longer and more accurate, inputs to another iteration of the recursive construction procedure; the entire process repeats until an error-free target molecule is formed. The method allows combining synthetic and natural DNA fragments into error-free designer DNA libraries, thus providing a foundation for the design and construction of complex synthetic DNA assemblies.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Yehezkel TB,Linshiz G,Kaplan S,Gronau I,Ravid S,Adar R,Shapiro Edoi
10.1016/B978-0-12-385120-8.00010-3subject
Has Abstractpub_date
2011-01-01 00:00:00pages
207-45eissn
0076-6879issn
1557-7988pii
B978-0-12-385120-8.00010-3journal_volume
498pub_type
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