Abstract：
:Recent advances have demonstrated the feasibility and robustness of chemical synthesis for the production of homogeneously glycosylated protein forms (glycoforms). By taking advantage of the unmatchable flexibility and precision provided by chemical synthesis, the quantitative effects of glycosylation were obtained using chemical glycobiology approaches. These findings greatly advanced our fundamental knowledge of glycosylation. More importantly, analysis of these findings has led to the development of glycoengineering guidelines for rationally improving the properties of peptides and proteins. In this chapter, we present the key experimental steps for chemical biology studies of protein glycosylation, with the aim of facilitating and promoting research in this important but significantly underexplored area of biology.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Li Y,Tran AH,Danishefsky SJ,Tan Zdoi
10.1016/bs.mie.2019.02.030subject
Has Abstractpub_date
2019-01-01 00:00:00pages
213-229eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30049-7journal_volume
621pub_type
杂志文章abstract：:Linkers play essential roles in the engineering of fusion proteins, and have been extensively demonstrated to affect protein properties such as expression level, solubility, and biological functions. For linker design and optimization, one of the key factors is the flexibility or rigidity of linkers, which describes t...
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journal_title：Methods in enzymology
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更新日期：2012-01-01 00:00:00
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journal_title：Methods in enzymology
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journal_title：Methods in enzymology
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pub_type： 杂志文章
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