Abstract:
:Coat protein complex II (COPII) vesicle-mediated protein export from the endoplasmic reticulum (ER) can be controlled by linear, independent motifs embedded within the cargo. ER export motifs directly interact with selective components of COPII vesicles and enhance cargo recruitment onto COPII vesicles. Moreover, ER export motifs are able to confer their transport abilities to other proteins. We have recently identified a novel ER export motif for α(2B)-adrenergic receptor (α(2B)-AR). This motif selectively interacts with Sec24C/D isoforms of COPII vesicles and facilitates α(2B)-AR export from the ER as well as transport to the cell surface. This motif can also mediate CD8 glycoprotein transport. These studies indicate that ER export of nascent G protein-coupled receptors (GPCRs) may be directed by specific codes that mediate receptor interaction with the ER-derived COPII vesicles. In this chapter, I discuss experimental approaches to identify ER export motifs for GPCRs by using α(2B)-AR as a model.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Wu Gdoi
10.1016/B978-0-12-391862-8.00010-7subject
Has Abstractpub_date
2013-01-01 00:00:00pages
189-202eissn
0076-6879issn
1557-7988pii
B978-0-12-391862-8.00010-7journal_volume
521pub_type
杂志文章abstract::Sedimentation velocity experiments measure the transport of molecules in solution under centrifugal force. Here, we describe a method for monitoring the sedimentation of very large biological molecular assemblies using the interference optical systems of the analytical ultracentrifuge. The mass, partial-specific volum...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2015.05.007
更新日期:2015-01-01 00:00:00
abstract::Enzymes in the α-d-phosphohexomutase (PHM) superfamily catalyze a multistep reaction, entailing two successive phosphoryl transfers. Key to this reaction is a conserved phosphoserine in the active site, which serves alternately as a phosphoryl donor and acceptor during the catalytic cycle. In addition to its role in t...
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章,评审
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abstract::Neurons are probably the most highly polarized cell type and typically develop a single axon and several dendrites. The establishment of a polarized morphology and the functional specialization of axonal and dendritic compartments are essential steps in the differentiation of neurons. Primary cultures of dissociated h...
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abstract::Guanine nucleotide exchange factors (GEFs) are essential regulators of the spatiotemporal conditions of small GTP-binding protein (SMG) activation. Their cellular activities combine the biochemical stimulation of GDP/GTP exchange, which leads to the active conformation of the SMG, to the detection of upstream signals ...
journal_title:Methods in enzymology
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pub_type: 杂志文章
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
pub_type: 杂志文章
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abstract::Portable, nanoparticle (NP)-enhanced enzyme sensors have emerged as powerful devices for qualitative and quantitative analysis of a variety of analytes for biomedicine, environmental applications, and pharmaceutical fields. This chapter describes a method for the fabrication of a portable, paper-based, inexpensive, ro...
journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/bs.mie.2016.03.006
更新日期:2016-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/S0076-6879(05)07032-1
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pub_type: 杂志文章
doi:10.1016/S0076-6879(09)05203-3
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journal_title:Methods in enzymology
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doi:10.1016/S0076-6879(05)94006-8
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journal_title:Methods in enzymology
pub_type: 杂志文章
doi:10.1016/B978-0-12-800280-3.00011-6
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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journal_title:Methods in enzymology
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更新日期:2006-01-01 00:00:00
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journal_title:Methods in enzymology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Methods in enzymology
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