Abstract:
:Astrocytes are the most abundant cell type in the brain and are a crucial part of solving its mysteries. Originally assumed to be passive supporting cells, astrocyte's functions are now recognized to include active modulation and information processing at the neural synapse. The full extent of the astrocyte contribution to neural processing remains unknown. This is, in part, due to the lack of methods available for astrocyte identification and analysis. Existing strategies employ genetic tools like the astrocyte specific promoters glial fibrillary acidic protein (GFAP) or Aldh1L1 to create transgenic animals with fluorescently labeled astrocytes. Recently, small molecule targeting moieties have enabled the delivery of bright fluorescent dyes to astrocytes. Here, we review methods for targeting astrocytes, with a focus on a recently developed methylpyridinium targeting moiety's development, chemical synthesis, and elaboration to provide new features like light-based spatiotemporal control of cell labeling.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Preston AN,Cervasio DA,Laughlin STdoi
10.1016/bs.mie.2019.02.006subject
Has Abstractpub_date
2019-01-01 00:00:00pages
129-151eissn
0076-6879issn
1557-7988pii
S0076-6879(19)30025-4journal_volume
622pub_type
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