Construction of immunotoxins using Pseudomonas exotoxin A.

Abstract:

:The above methodology has been used to prepare a variety of PE-immunotoxins. These reagents are potent cytotoxic agents for cells in culture that bind the appropriate antibody and nontoxic when cells do not bind the antibody. PE-immunotoxins can be used to select for mutant cultured cells which lack the target of the antibody (see chapter by Gottesman [9] on drug-resistant mutants). Recently, we have also demonstrated in vivo activity when a PE-immunotoxin was used to inhibit the growth of human ovarian cancer cells in a nude mouse model of ovarian cancer. Also PE-immunotoxins are currently being evaluated in Phase 1 clinical trials for the treatment of adult T-cell leukemia.

journal_name

Methods Enzymol

journal_title

Methods in enzymology

authors

FitzGerald DJ

doi

10.1016/s0076-6879(87)51015-1

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

139-45

eissn

0076-6879

issn

1557-7988

pii

S0076-6879(87)51015-1

journal_volume

151

pub_type

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