Abstract：
:Autophagy is the major cellular process of degradation and is modulated by several signaling pathways. Phosphatidylinositol 3-kinase (PtdIns3K) class III (Vps34) and PtdIns3K class I regulate the autophagy pathway positively and negatively, respectively. Both classes of PtdIns3K participate in the synthesis of phosphatidylinositol 3-phosphate (PtdIns3P), which plays a crucial role in autophagosome biogenesis and membrane traffic. PtdIns3P is a membrane phospholipid that is associated with endogenous FYVE domain-containing proteins. Indeed, such interactions facilitate autophagosome fusion with lysosomes and subsequent cargo degradation. During starvation-induced autophagy, the expression of FYVE domain-containing proteins increases, and their binding to PtdIns3P is strengthened. Nonetheless, not all FYVE domain proteins are related to the induction of autophagy. This method report presents the quantification of PtdIns3P synthesis by using cells either transiently transfected with or stably expressing FYVE-dsRed.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Yakhine-Diop SM,Martínez-Chacón G,González-Polo RA,Fuentes JM,Niso-Santano Mdoi
10.1016/bs.mie.2016.09.060subject
Has Abstractpub_date
2017-01-01 00:00:00pages
257-269eissn
0076-6879issn
1557-7988pii
S0076-6879(16)30333-0journal_volume
587pub_type
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