Rat glioma cell death induced by cationic liposome-mediated transfer of the herpes simplex virus thymidine kinase gene followed by ganciclovir treatment.

Abstract:

BACKGROUND AND OBJECTIVES:We studied antitumor effects and cell death induced by cationic liposome-mediated gene transfer of the herpes simplex virus thymidine kinase (HSV-tk) gene followed by ganciclovir treatment in cultured rat T9 glioma cells and in experimental gliomas produced from this cell line. METHODS:To transfer genes we used small unilamellar cationic liposomes containing N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride. Video-enhanced contrast differential interference contrast microscopy was used for morphologic observations of cultured cells. RESULTS:When we treated the cells or implanted gliomas with the liposomes and ganciclovir, a strong effect was seen against tumor cells, and survival of tumor-implanted rats was increased. Morphologically, cell death observed after HSV-tk gene/liposome and ganciclovir treatment in the cultured glioma cells included both apoptosis and necrosis. CONCLUSIONS:Introduction of the HSV-tk gene in a DNA-liposome complex followed by ganciclovir treatment induced both apoptosis and necrosis, which together resulted in a potent antitumor effect.

journal_name

J Surg Oncol

authors

Yoshida T,Mizuno M,Taniguchi K,Nakayashiki N,Wakabayashi T,Yoshida J

doi

10.1002/1096-9098(200101)76:1<19::aid-jso1004>3.0.

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

19-25

issue

1

eissn

0022-4790

issn

1096-9098

pii

10.1002/1096-9098(200101)76:1<19::AID-JSO1004>3.0.

journal_volume

76

pub_type

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