Comparison of three urease tests for detection of Helicobacter pylori in gastric biopsy specimens.

Abstract:

OBJECTIVE:To propose two modified urease tests for the detection of Helicobacter pylori in gastric biopsy specimens. PATIENTS AND METHODS:The presence of H. pylori infection was determined in 237 patients undergoing upper gastrointestinal endoscopy. Three media were used for the urease tests: Christensen's 2% urea broth and two urea agar media, modified by increasing the concentration of urea (to 4% and 10%) and phenol red and omitting the nutrients. RESULTS:The modified tests had good sensitivity (> 78%), specificity (96%) and accuracy (> or = 86%) at 2 h using small amounts (15%) of biopsy homogenates. They were statistically more sensitive and accurate than Christensen's broth. CONCLUSION:Both the modified 4% and 10% urea agar tests are simple, sensitive and specific and can be performed with small amounts of sample.

authors

Boyanova L,Stancheva I,Todorov D,Kumanova R,Petrov S,Vladimirov B,Pehlivanov N,Mitova R,Chakarski I,Churchev I

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

911-4

issue

9

eissn

0954-691X

issn

1473-5687

journal_volume

8

pub_type

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