Infrequent p16/CDKN2 alterations in squamous cell carcinoma of the oesophagus.

Abstract:

:Loss of heterozygosity (LOH) on chromosome 9 and p16 (MTS1/CDKN2) gene mutations have been reported in various human cancers. The present study aimed to determine the prevalence of LOH in 100 oesophageal squamous cell carcinomas (OSCCs) by typing microsatellite loci and mutations of the p16 gene. The methods used included denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of exon 2. LOH was found in 14.7% of the OSCC cases. Six gene alterations were identified in exon 2. They consisted of three deletions and the same polymorphism in three samples. The relatively low rate of p16 mutation compared with the frequency of LOH suggests the possible involvement of another tumour suppressor gene located on chromosome 9 in oesophageal carcinogenesis.

authors

Giroux MA,Audrezet MP,Metges JP,Lozac'h P,Volant A,Nousbaum JB,Labat JP,Gouérou H,Ferec C,Robaszkiewicz M

doi

10.1097/00042737-200201000-00004

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

15-8

issue

1

eissn

0954-691X

issn

1473-5687

journal_volume

14

pub_type

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