Huperzine A ameliorates the impaired memory of aged rat in the Morris water maze performance.

abstract：AIM:Hydrogen peroxide (H2O2) is produced during liver transplantation. Ischemia/reperfusion induces oxidation and causes intracellular Ca2+ overload, which harms liver cells. Our goal was to determine the precise mechanisms of these processes. METHODS:Hepatocytes were extracted from rats. Intracellular Ca2+ concentrat...

journal_title：Acta pharmacologica Sinica

authors： Sato H,Takeo T,Liu Q,Nakano K,Osanai T,Suga S,Wakui M,Wu J

更新日期：2009-01-01 00:00:00

abstract：AIM:To evaluate the efficiency of 3 short hairpin RNA (shRNA) interfering with the herpes simplex virus type 1 (HSV-1) gene coding glycoprotein D (gD) for inhibiting the gD expression and virus replication in vitro. METHODS:Vero cells were selected for an in vitro model of infection. Three shRNA sequences (shRNAgD1, -...

journal_title：Acta pharmacologica Sinica

authors： Liu YY,Deng HY,Yang G,Jiang WL,Grossin L,Yang ZQ

更新日期：2008-08-01 00:00:00

abstract：AIM:To model the three-dimensional structure and investigate the interaction mechanism of the proprotein convertase furin/kexin and their inhibitors (eglin c mutants). METHODS:The three-dimensional complex structures of furin/kexin with its inhibitors, eglin c mutants, were generated by modeller program using the newl...

journal_title：Acta pharmacologica Sinica

authors： Cai XH,Zhang Q,Ding DF

更新日期：2004-12-01 00:00:00

abstract：AIM:To prepare neutralizing monoclonal antibody (mAb) against extracellular immunoglobulin (Ig)-like domain III of vascular endothelial growth factor receptor KDR and study its biological activity. METHODS:Soluble KDR Ig domain III (KDR-III) fusion protein was expressed in E Coli and purified from the bacterial peripl...

journal_title：Acta pharmacologica Sinica

authors： Li R,Xiong DS,Shao XF,Liu J,Xu YF,Xu YS,Liu HZ,Zhu ZP,Yang CZ

更新日期：2004-10-01 00:00:00

abstract：:Diabetic nephropathy (DN) is characterized by sterile inflammation with continuous injury and loss of renal inherent parenchyma cells. Podocyte is an essential early injury target in DN. The injury and loss of podocytes are closely associated with proteinuria, the early symptom of renal injury in DN. However, the exac...

journal_title：Acta pharmacologica Sinica

authors： Cheng Q,Pan J,Zhou ZL,Yin F,Xie HY,Chen PP,Li JY,Zheng PQ,Zhou L,Zhang W,Liu J,Lu LM

更新日期：2020-09-23 00:00:00

abstract：AIM:The role of caveolin-1 (Cav-1) in angiogenesis remains poorly understood. The endothelial nitric oxide (NO) synthase (eNOS), a caveolin-interacting protein, was demonstrated to play a predominant role in vascular endothelial growth factor (VEGF) -induced angiogenesis. The purpose of our study was to examine the rol...

journal_title：Acta pharmacologica Sinica

authors： Pan YM,Yao YZ,Zhu ZH,Sun XT,Qiu YD,Ding YT

更新日期：2006-12-01 00:00:00

abstract：AIM:To investigate the effects of salvianolate, a water-soluble active compound from Salvia miltiorrhiza Bunge, on reactive oxygen species (ROS) production in mouse cardiomyocytes in vitro. METHODS:Primary ventricular cardiomyocytes were prepared from neonatal mouse. The cell viability was determined using MTT assay. ...

journal_title：Acta pharmacologica Sinica

authors： Fei AH,Cao Q,Chen SY,Wang HR,Wang FL,Pan SM,Lin ZF

更新日期：2013-04-01 00:00:00

abstract：AIM:To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. METHODS:Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assist...

journal_title：Acta pharmacologica Sinica

authors： Li ZJ,Liu N,Han QD,Zhang YY

更新日期：2011-09-01 00:00:00

abstract：:Activation of the Ca2+/calmodulin-dependent protein kinase II isoform δA (CaMKIIδA) disturbs intracellular Ca2+ homeostasis in cardiomyocytes during chronic heart failure (CHF). We hypothesized that upregulation of CaMKIIδA in cardiomyocytes might enhance Ca2+ leak from the sarcoplasmic reticulum (SR) via activation o...

journal_title：Acta pharmacologica Sinica

authors： Gui L,Guo X,Zhang Z,Xu H,Ji YW,Wang RJ,Zhu JH,Chen QH

更新日期：2018-10-01 00:00:00

abstract：AIM:To examine whether the cell growth inhibitory effect of the combination of baicalin and baicalein is related to apoptosis. Moreover, to determine whether the expression of some apoptosis-related proteins is regulated by the ERK/p38 MAPK pathway. METHODS:Cell viability was measured using a 3-(4,5-dimethylthiazol-2-...

journal_title：Acta pharmacologica Sinica

authors： Zhou QM,Wang S,Zhang H,Lu YY,Wang XF,Motoo Y,Su SB

更新日期：2009-12-01 00:00:00

abstract：:In an effort to develop effective alternative strategies that increase the therapeutic efficacy and minimize the systemic toxicity of chemotherapeutic agents, more efforts are being directed towards the investigation of dietary supplements and other phytotherapeutic agents for their synergistic efficacy in combination...

journal_title：Acta pharmacologica Sinica

authors： Raina K,Agarwal R

更新日期：2007-09-01 00:00:00

abstract：:Midbrain dopaminergic (DA) neurons are governed by an endogenous cholinergic system, originated in the mesopontine nuclei. Nicotine hijacks nicotinic acetylcholine receptors (nAChRs) and interferes with physiological function of the cholinergic system. In this review, we describe the anatomical organization of the cho...

journal_title：Acta pharmacologica Sinica

authors： Xiao C,Zhou CY,Jiang JH,Yin C

更新日期：2020-01-01 00:00:00

abstract：AIM:To study the antioxidant, activity of Qizhu Tang (QZT) both in vivo and in vitro. METHODS:QZT consists of 4 herbal constituents (Rhizoma Atractylodis Macrocephalae, Poria cocos, Kadix Notoginseng, and Radix Astragali), each of the components and their combinations were examined in vitro for 1, 1-diphenyl-2-picrylh...

journal_title：Acta pharmacologica Sinica

authors： Wang XJ,Feng P

更新日期：2000-12-01 00:00:00

abstract：AIM:To investigate the inhibitory effect of the natural product Leukamenin F on liver fibrosis and explore its potential underlying mechanisms. METHODS:Carbon tetrachloride (CCl(4))-treated mouse model in vivo and in hepatic stellate cells (HSC) in vitro were used. The effect on CCl(4)-induced liver fibrosis was studi...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Wang X,Zhang Y,Li CJ,Hu LH,Shen X

更新日期：2010-07-01 00:00:00

abstract：AIM:To study the synaptic mechanisms involved in the 5-hydroxytryptamine1A/7 (5-HT1A/7) receptor-mediated reflex control of cardiac vagal preganglionic neurons (CVPN). METHODS:CVPN were retrogradely labeled and identified in brain stem slices of newborn rats, and their synaptic activity was examined using whole-cell p...

journal_title：Acta pharmacologica Sinica

authors： Chen YH,Hou LL,Wang JJ

更新日期：2008-05-01 00:00:00

abstract：AIM:To investigate the effects of trans-cinnamaldehyde (TCA) on the human leukemia K562 cell line and the cytotoxicity of cytokine-induced killer (CIK) cells against K562 cells. METHODS:Apoptosis, Fas expression, and mitochondrial transmembrane potential in K652 cells were analyzed using flow cytometry. K562 cells wer...

journal_title：Acta pharmacologica Sinica

authors： Zhang JH,Liu LQ,He YL,Kong WJ,Huang SA

更新日期：2010-07-01 00:00:00

abstract：AIM:Catechol estrogens and 16alpha-hydroxy estrogen are important metabolites that cause carcinogenesis. This study was aimed to stud y the role of cytochrome P450 in estradiol metabolism. METHODS:The estradiol metabolites were determined with HPLC-ECD. Correlation of estradiol metabolites production between cytochrom...

journal_title：Acta pharmacologica Sinica

authors： Cheng ZN,Shu Y,Liu ZQ,Wang LS,Ou-Yang DS,Zhou HH

更新日期：2001-02-01 00:00:00

abstract：AIM:To develop a simple method for monitoring protein localization of epidermal growth factor (EGF) in living cells. METHODS:Enhanced green fluorescent protein (EGFP) was used as an autofluorescent tag to label EGF ligands. SDS-PAGE and Western blot analysis were used to detect the expression of the EGFP-tagged EGF (E...

journal_title：Acta pharmacologica Sinica

authors： Jiang H,Zhang J,Shi BZ,Xu YH,Li ZH,Gu JR

更新日期：2007-01-01 00:00:00

abstract：AIM:To investigate the effects of 3,4,4'-trihydroxy-trans-stilbene (3,4,4'-THS), an analogue of resveratrol, on human non-small-cell lung cancer (NSCLC) cells in vitro. METHODS:Cell viability of NSCLC A549 cells was determined by MTT assay. Cell apoptosis was evaluated using flow cytometry and TUNEL assay. Cell necros...

journal_title：Acta pharmacologica Sinica

authors： Zhang L,Dai F,Sheng PL,Chen ZQ,Xu QP,Guo YQ

更新日期：2015-10-01 00:00:00

abstract：AIM:To study in vitro release and in vivo effect of four different types of sustained-release naltrexone microspheres on morphine analgesia. METHODS:Release of naltrexone from four types of biodegradable microspheres was investigated by HPLC. Their antagonist effects on morphine analgesia were observed using mouse hot...

journal_title：Acta pharmacologica Sinica

authors： He GA,Hou HM,Liu XJ

更新日期：2001-06-01 00:00:00

abstract：:Central nervous system (CNS) disorders represent a broad spectrum of brain ailments with short- and long-term disabilities, and nanomedicine-based approaches provide a new therapeutic approach to treating CNS disorders. A variety of potential drugs have been discovered to treat several neuronal disorders; however, the...

journal_title：Acta pharmacologica Sinica

authors： Hanif S,Muhammad P,Chesworth R,Rehman FU,Qian RJ,Zheng M,Shi BY

更新日期：2020-07-01 00:00:00

abstract：AIM:To compare high K(+ )-induced contraction and optimal resting tension measured by two commonly used techniques of hypertonic and isotonic K(+ ) in aortas with and without adventitial fat from various age rats. METHODS:Three age groups of rats (15, 25, and 62 weeks) were used to prepare thoracic aortic rings in whi...

journal_title：Acta pharmacologica Sinica

authors： Guan YF,Chen RH,Wang P,Qin Y,Su DF,Miao CY

更新日期：2007-05-01 00:00:00

abstract：AIM:To investigate the effect of epigallocatechin gallate (EGCG) on angiotensin II (Ang II)-induced stress fiber formation and hyperpermeability in endothelial cells. METHODS:Human umbilical vein endothelial cells (HUVECs) were treated with Ang II in the absence or presence of EGCG or mitogen-activated protein kinases...

journal_title：Acta pharmacologica Sinica

authors： Yang D,Liu J,Tian C,Zeng Y,Zheng YH,Fang Q,Li HH

更新日期：2010-10-01 00:00:00

abstract：AIM:Cathepsin L is a lysosomal cysteine protease that plays important roles in cancer tumorigenesis, proliferation and chemotherapy resistance. The aim of this study was to determine how cathepsin L regulated the radiosensitivity of human glioma cells in vitro. METHODS:Human glioma U251 cells (harboring the mutant typ...

journal_title：Acta pharmacologica Sinica

authors： Zhang QQ,Wang WJ,Li J,Yang N,Chen G,Wang Z,Liang ZQ

更新日期：2015-09-01 00:00:00

abstract：AIM:To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes. METHODS:Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, th...

journal_title：Acta pharmacologica Sinica

authors： Ji XH,Yao T,Qin JC,Wang SK,Wang HJ,Yao K

更新日期：2004-10-01 00:00:00

abstract：AIM:The mechanism of tramadol-induced vasodilation was investigated using isolated rabbit thoracic aortic rings. METHODS:Aortic rings from 8 rabbits were placed in organ bath and precontracted with phenylephrine (10(-5) mol/L) before addition of tramadol. Relaxation responses by tramadol were evaluated in the presence...

journal_title：Acta pharmacologica Sinica

authors： Kaya T,Gursoy S,Karadas B,Sarac B,Fafali H,Soydan AS

更新日期：2003-05-01 00:00:00

abstract：AIM:To evaluate the protective effects of apocynin on "two-hit" injury in rats. METHODS:"Two-hit" injury model of rat was induced by hemorrhagic shock (40 mmHg for 45 min) followed by iv administration of lipopolysaccharide (LPS, 150 microg/kg). Rats were randomized into seven groups: Sham, LPS, hemorrhage, hemorrhage...

journal_title：Acta pharmacologica Sinica

authors： Zhou R,Hu DY,Liu LM,Zhou XW

更新日期：2002-11-01 00:00:00

abstract：AIM:To examine if steroid-like compounds found in many Chinese medicinal products conventionally used for the promotion of blood circulation may act as active components via the same molecular mechanism triggered by cardiac glycosides, such as ouabain. METHODS:The inhibitory potency of ouabain and the identified stero...

journal_title：Acta pharmacologica Sinica

authors： Chen RJ,Chung TY,Li FY,Yang WH,Jinn TR,Tzen JT

更新日期：2010-06-01 00:00:00

abstract：:For follicular lymphoma (FL) with grade 1/2, the complete response (CR) rate of the first-line R-CHOP treatment was significantly low. In this study, we assessed the rationality of the administration of rituximab for FL patients with grade 1/2 based on concentration-response relationship analyses. Thus, we conducted a...

journal_title：Acta pharmacologica Sinica

authors： Liu S,Huang H,Chen RX,Wang Z,Guan YP,Peng C,Fang XJ,Chen ZJ,Guan SX,Zhu X,Ren QG,Yao YY,Huang HB,Huang M,Wang XD,Lin TY

更新日期：2020-07-31 00:00:00

abstract：AIM:To investigate the effects and underlying mechanisms of 118, a novel derivative of mycophenolic acid, in a murine allogeneic skin graft model. METHODS:Skin grafts were conducted by grafting BALB/c donor tail skin into C57BL/6 skin beds (allograft) or by grafting female C57BL/6 donor tail skin into female C57BL/6 s...

journal_title：Acta pharmacologica Sinica

authors： Kong FY,Chen W,He SJ,Lin ZM,Li X,Zhang XH,Yang XQ,Zhu FH,Tong XK,Zhou Y,Tang W,Duan WH,Zuo JP

更新日期：2013-07-01 00:00:00