Abstract:
:The role of bicarbonate as a hydrogen ion buffer has been investigated using the fluorescent dye BCECF in individual rat cerebellar, hippocampal and neocortical neurones maintained in culture. The steady-state intracellular pH (pHi) was estimated to be 7.07 +/- 0.05 (n = 22) in CO2-HCO3(-)-buffered media. Buffering power (beta) estimated from the addition and removal of weak bases was ca 10 mM (pH unit)-1 and was found to be similar in both CO2-HCO3(-)- and Hepes-buffered media. The membrane-permeant carbonic anhydrase inhibitor, acetazolamide (10-20 microM), did not affect estimates of beta. The results indicate that CO2-HCO3- does not act as an open buffer system in these neurones.
journal_name
Exp Physioljournal_title
Experimental physiologyauthors
Amos BJ,Pocock G,Richards CDdoi
10.1113/expphysiol.1996.sp003963subject
Has Abstractpub_date
1996-07-01 00:00:00pages
623-32issue
4eissn
0958-0670issn
1469-445Xjournal_volume
81pub_type
杂志文章abstract::Disrupting circadian rhythms in rodents perturbs glucose metabolism and increases adiposity. To determine whether these effects occur in a large diurnal animal, we assessed the impact of circadian rhythm disruption upon metabolic function in sheep. Adult ewes (n = 7) underwent 3 weeks of a control 12 h light-12 h dark...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2014.080630
更新日期:2014-09-01 00:00:00
abstract::Obstructive sleep apnoea (OSA) leads to chronic intermittent hypoxia (CIH) during sleep. Obstructive sleep apnoea has been associated with liver injury. Acetaminophen (APAP; known as paracetamol outside the USA) is one of the most commonly used drugs which has known hepatotoxicity. The goal of the present study was to...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2008.044883
更新日期:2009-02-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Pregnancy requires a robust plasma volume expansion driven by renal sodium retention. In the late-pregnant kidney, the aldosterone-responsive epithelial Na(+) channel is increased, whereas the sodium-chloride cotransporter is decreased. Pendrin has been shown to ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP085396
更新日期:2015-10-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Does 14 days of live-high, train-low simulated altitude alter an individual's metabolomic/metabolic profile? What is the main finding and its importance? This study demonstrated that ∼200 h of moderate simulated altitude exposure resulted in greater variance in m...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087159
更新日期:2019-01-01 00:00:00
abstract::Cessation of milk removal causes mammary tissue involution, which in rodents is characterized by extensive tissue degeneration and loss of the majority of luminal epithelial cells by apoptosis. In contrast, bovine mammary tissue shows little histological evidence of tissue remodelling between lactations. In this study...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1997.sp004075
更新日期:1997-09-01 00:00:00
abstract::The effects of muscle fatigue on the mechanical efficiency of muscle work performance were studied in situ, in stretch-shortening cycles that resembled physiological conditions. The experiments were performed on the medial gastrocnemius muscles of seven rats. To calculate efficiency, a simple Hill-type model was used,...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1997.sp004001
更新日期:1997-01-01 00:00:00
abstract:NEW FINDINGS:What is the topic of this review? This brief review discusses potential sex differences in arterial function across the age span, with special emphasis on the effects of oestrogen and testosterone on the vascular endothelium. What advances does it highlight? We discuss the relationship between the impacts ...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP085367
更新日期:2016-02-01 00:00:00
abstract::A video-laryngoscopic method, implemented with an algorithm for the correction of the deformation inherent in the endoscope optical system, has been used to measure the dorsoventral diameter (Drg) and the cross-sectional area (CSArg) of the rima glottidis in five healthy workhorses during conscious breathing at rest. ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Despite its critical role in restoring cardiac rhythm and thus in saving human life, cardiac defibrillation remains poorly understood. Further mechanistic inquiry is hampered by the inability of presently available experimental techniques to resolve, with sufficient accuracy, electrical behaviour confined to the depth...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/expphysiol.2005.030973
更新日期:2006-03-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/expphysiol.2005.032854
更新日期:2007-03-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP086083
更新日期:2017-06-01 00:00:00
abstract::Embryonic stem (ES) cells are pluripotent cells isolated from the inner cell mass of blastocysts. ES cells are able to differentiate into the three primitive layers (endoderm, mesoderm and ectoderm) of the organism, including the germline. To study early stages of development, as well as to investigate the impact of a...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:
更新日期:2000-11-01 00:00:00
abstract::The effects of microinjections of adenosine and an adenosine receptor antagonist into the rostral ventrolateral medulla (RVLM) on the cardiovascular changes associated with the defence reaction were investigated in anaesthetized rats. Responses to electrical and chemical stimulation in RVLM were determined in alpha-ch...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1996.sp003919
更新日期:1996-01-01 00:00:00
abstract::The autonomic adjustments to exercise are mediated by central signals from the higher brain (central command) and by a peripheral reflex arising from working skeletal muscle (exercise pressor reflex), with further modulation provided by the arterial baroreflex. Although it is clear that central command, the exercise p...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/expphysiol.2005.032037
更新日期:2006-01-01 00:00:00
abstract::What is the central question of this study? Myocardial ischaemia-reperfusion (I/R) injury causes interference in the systemic circulation and damages not only the heart but also several vital organs, including the brain. Recently, a novel peptide called humanin has been shown to exert potent neuroprotective effects. H...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP085749
更新日期:2016-06-01 00:00:00
abstract::This study investigated the role of nitric oxide (NO) and superoxide anions in modulating the renal nerve-dependent increases in proximal tubular fluid reabsorption (Jva). Renal nerve stimulation at 0.75 and 1.0 Hz (15 V, 0.2 ms) in anaesthetized Wistar rats had no effect on glomerular filtration rate but decreased ur...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2003.002640
更新日期:2004-05-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087449
更新日期:2019-07-01 00:00:00
abstract::Contractile properties and fibre type composition of slow-twitch soleus muscles from spontaneously hypertensive rats (SHR) were compared with those from a control normotensive strain (Wistar-Kyoto, WKY). Male rats aged around 100 days were used, and muscle tension was recorded isometrically in vitro. Tetanic tension w...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1994.sp003772
更新日期:1994-05-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? We modelled the alveolar pathway during breath holding on the hypothesis that it follows a hypoventilation loop on the O2 -CO2 diagram. What is the main finding and its importance? Validation of the model was possible within the range of alveolar gas compositions...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP088977
更新日期:2020-09-29 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Does the combination of methazolamide and theophylline reduce symptoms of acute mountain sickness (AMS) and improve aerobic performance in acute hypobaric hypoxia? What is the main finding and its importance? The oral combination of methazolamide (100 BID) and th...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP088461
更新日期:2021-01-01 00:00:00
abstract::An energy-rich diet leads to enhanced ruminal Na(+) absorption, which is associated with elevated plasma insulin-like growth factor 1 (IGF-1) levels and an increased number of IGF-1 receptors in rumen papillae. This study examined the in vitro effect of IGF-1 on Na(+) transport across the rumen epithelium of hay-fed s...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2011.061580
更新日期:2012-04-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP086846
更新日期:2018-08-01 00:00:00
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journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2010.054031
更新日期:2010-12-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Regulation of cardiac function is typically achieved by changes in heart rate (HR) and cardiac shortening velocity (strain rate; SR), but their interdependence in vivo remains poorly understood. What is the main finding and its importance? Using resistance exerci...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP085081
更新日期:2015-04-20 00:00:00
abstract::Vasopressin and oxytocin release from the neural lobe, and the vasopressin and oxytocin mRNA contents of the supraoptic and paraventricular nuclei are increased by hypertonicity of the extracellular fluid. The factors regulating these parameters can be conveniently studied in perifused explants of the hypothalamo-neur...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1111/j.1469-445x.2000.tb00021.x
更新日期:2000-03-01 00:00:00
abstract::Salivary fluid secretion is dependent upon reflex stimuli mediated by autonomic nerves. In order to determine if immunoglobulin A (IgA) and salivary proteins are secreted in the absence of nerve stimulation, small volumes (< 2 microl) of saliva were consecutively collected from the submandibular duct of anaesthetised ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/eph8802438
更新日期:2003-01-01 00:00:00
abstract::Rats were fed a liquid diet with the aim of decreasing nervous reflex activity in the parotid glands. In rats killed after 21 days on this diet the glands were atrophied and the total amounts of the neuropeptides, substance P, vasoactive intestinal peptide and calcitonin gene-related peptide, were lower than in contro...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1990.sp003435
更新日期:1990-07-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Compared with Caucasians, African individuals are more susceptible to non-freezing cold injury and experience greater cutaneous vasoconstriction and cooler finger skin temperatures upon hand cooling. We investigated whether the enzyme cyclooxygenase is, in part, ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP086186
更新日期:2017-07-01 00:00:00
abstract::Methyl CpG binding protein-2 (MECP2) is a chromatin-remodelling factor with a dual role in gene expression. Evidence from patients carrying MECP2 mutations and from transgenic mouse models demonstrates that this protein is involved in the control of body weight. However, the mechanism for this has not been fully eluci...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2014.079798
更新日期:2014-09-01 00:00:00
abstract::Aortic bodies are arterial chemoreceptors presumed to monitor blood O2 content by unknown mechanisms, in contrast to their well-studied carotid body counterparts, which monitor PO2 and /pH. We recently showed that rat aortic body chemoreceptors (type I cells), located at the left vagus-recurrent laryngeal nerve bifurc...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2013.075408
更新日期:2014-01-01 00:00:00