Abstract:
:Methyl CpG binding protein-2 (MECP2) is a chromatin-remodelling factor with a dual role in gene expression. Evidence from patients carrying MECP2 mutations and from transgenic mouse models demonstrates that this protein is involved in the control of body weight. However, the mechanism for this has not been fully elucidated. To address this, we used a previously characterized Mecp2-null mouse model and found that the increase in body weight is associated with an increased amount of adipose tissue and high leptin levels. Appropriate body weight control requires the proper expression of pro-opiomelanocortin (Pomc) and agouti-related peptide (Agrp), two neuropeptides essential for satiety and appetite signals, respectively. Our results show that in the absence of Mecp2, Pomc and Agrp mRNA expression are altered, and the mice are leptin resistant. To determine the mechanism underlying the defective leptin sensing, we evaluated the expression of genes and the post-translational modifications associated with leptin signalling, which are fundamental to Pomc and Agrp transcriptional control and proper leptin response. We found a decrease in the phosphorylation level of Akt and its target protein Foxo1, which indicate an alteration in leptin-induced signal transduction. Our results demonstrate that the absence of Mecp2 disrupted body weight balance by altering post-translational modifications in leptin-signalling components that regulate Pomc and Agrp expression.
journal_name
Exp Physioljournal_title
Experimental physiologyauthors
Torres-Andrade R,Moldenhauer R,Gutierrez-Bertín N,Soto-Covasich J,Mancilla-Medina C,Ehrenfeld C,Kerr Bdoi
10.1113/expphysiol.2014.079798subject
Has Abstractpub_date
2014-09-01 00:00:00pages
1229-40issue
9eissn
0958-0670issn
1469-445Xpii
expphysiol.2014.079798journal_volume
99pub_type
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