Dietary pectin up-regulates monocaboxylate transporter 1 in the rat gastrointestinal tract.

Abstract:

:This work was undertaken to study the effect of pectin feeding on the expression level, cellular localization and functional activity of monocarboxylate transporter 1 (MCT1) in the gastrointestinal tract of rats. The results indicated that MCT1 protein level was significantly increased along the entire length of the gastrointestinal tract of pectin-fed rats in comparison with control animals. Immunohistochemical analysis revealed an increase in MCT1 in the stratified squamous epithelia of the forestomach as well as in the basolateral membranes of the cells lining the gastric pit of the glandular stomach of pectin-fed rats when compared with control animals. The parietal cells, which showed barely any or no detectable MCT1 in the control group, exhibited a strong intensity of MCT1 on the basolateral membranes in pectin-fed rats. In the small intestine of pectin-fed rats, strong immunopositivity for MCT1 was detected in the brush border and basolateral membranes of the absorptive enterocytes lining the entire villi, while in control rats, weak reactivity was detected on the brush border membrane in a few absorptive enterocytes in the villus tip. In the large intestine of control animals, MCT1 was detected on the basolateral membranes of the epithelia lining the caecum and colon. This staining intensity was markedly increased in pectin-fed rats, along with the appearance of strong reactivity for MCT1 on the apical membranes of the surface and crypt epithelia of caecum and colon. Our results also showed that MCT1 co-localizes with its chaperone, basigin (CD147), in the rat gastrointestinal tract, and that the pectin feeding increased the expression of CD147. In vivo functional studies revealed an enhanced acetate absorption in the colon of pectin-fed in comparison with control animals. We conclude that MCT1 is up-regulated along the gastrointestinal tract of pectin-fed rats, which might represent an adaptive response to the increased availability of its substrates.

journal_name

Exp Physiol

journal_title

Experimental physiology

authors

Kirat D,Kondo K,Shimada R,Kato S

doi

10.1113/expphysiol.2009.046797

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

422-33

issue

4

eissn

0958-0670

issn

1469-445X

pii

expphysiol.2009.046797

journal_volume

94

pub_type

杂志文章
  • Activation of the renin-angiotensin system contributes to the peripheral vasoconstriction reflexly caused by stomach distension in anaesthetized pigs.

    abstract::Gastric distension in anaesthetized pigs reflexly elicits peripheral vasoconstriction and an increase in plasma renin activity (PRA), with vagal afferent and sympathetic efferent limbs. The aim of the present study was to quantify the contribution of the renin-angiotensin system to the peripheral vasoconstriction. In ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/eph8802560

    authors: Molinari C,Battaglia A,Grossini E,Florio S,Mary DA,Vassanelli C,Vacca G

    更新日期:2003-05-01 00:00:00

  • The use of midbrain stimulation to identify the discharges of static and dynamic fusimotor neurones during reflex jaw movements in the anaesthetized cat.

    abstract::In anaesthetized cats, it was possible to enhance the dynamic sensitivity to stretch of jaw muscle spindle primary afferents by electrical stimulation in the midbrain. This effect was taken to be due to excitation of dynamic fusimotor neurones and was used to identify fusimotor fibres as static or dynamic in recording...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1993.sp003667

    authors: Donga R,Taylor A,Jüch PJ

    更新日期:1993-01-01 00:00:00

  • A proportional assist ventilator to unload respiratory muscles experimentally during exercise in humans.

    abstract::What is the central question of this study? Can a modern proportional assist ventilator (PAV) function sufficiently well to unload the respiratory muscles during exercise? What is the main finding and its importance? A PAV can be constructed with contemporary hardware and software and be used at all exercise intensiti...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP085735

    authors: Dominelli PB,Henderson WR,Sheel AW

    更新日期:2016-06-01 00:00:00

  • The gut hormone secretin triggers a gut-brown fat-brain axis in the control of food intake.

    abstract:NEW FINDINGS:What is the topic of this review? Brown fat's role in meal-associated thermogenesis and the related consequences for energy balance regulation with a focus on the gut hormone secretin, which has been identified as the endocrine molecular mediator of meal-associated brown fat thermogenesis. What advances do...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP087878

    authors: Schnabl K,Li Y,Klingenspor M

    更新日期:2020-08-01 00:00:00

  • Intracellular sodium activity in papillary muscle from diabetic rat hearts.

    abstract::In quiescent papillary muscles isolated from hearts of rats with streptozotocin-induced diabetes resting intracellular sodium activity (aiNa) was about 56% greater than in muscles from controls. An intracellular acid load induced by the NH4+ method caused a rise in aiNa whose maximum amplitude was similar in both grou...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1991.sp003478

    authors: Lagadic-Gossmann D,Feuvray D

    更新日期:1991-01-01 00:00:00

  • Differential effects of age and sex on the postnatal responsiveness of brown adipose tissue to prolactin administration in rats.

    abstract::Previous studies have shown that prolactin administration to pregnant rats results in offspring with enhanced abundance of the brown adipose tissue-specific uncoupling protein (UCP) 1. The present study therefore aimed to determine whether a similar effect was observed after birth and if the sex of the animal further ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/eph8802575

    authors: Pearce S,Dieguez C,Gualillo O,Symonds ME,Stephenson T

    更新日期:2003-07-01 00:00:00

  • The splanchnic anti-inflammatory pathway: could it be the efferent arm of the inflammatory reflex?

    abstract::What is the topic of this review? We review the current literature on the neural reflex termed the 'inflammatory reflex' that inhibits an excessive release of inflammatory mediators in response to an immune challenge. What advances does it highlight? The original model proposed that the inflammatory reflex is a vago-v...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/EP085559

    authors: Martelli D,Farmer DG,Yao ST

    更新日期:2016-10-01 00:00:00

  • Peroxidase and kallikrein in atropine-resistant secretion of submandibular saliva on parasympathetic nerve stimulation in anaesthetized rats.

    abstract::Flow of submandibular saliva and the constituents secreted during chorda-lingual nerve stimulation at 40 Hz (3 x 10 min) were studied after alpha- and beta-adrenergic blockade, and in the absence (control) or presence of cholinergic blockade in anaesthetized rats. Peroxidase and true tissue kallikrein (rK1) were asses...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1996.sp003940

    authors: Garrett JR,Anderson LC,Zhang XS,Proctor GB

    更新日期:1996-05-01 00:00:00

  • Chronic intermittent immobilization of male rats throughout sexual development: a stress protocol.

    abstract::A stress protocol--6 h of daily immobilization--was applied throughout male rat sexual development. Immobilization caused a small reduction in food intake and body weight gain whereas pair-fed animals had a marginal decrease only in body weight gain. Stress, confirmed by increased plasma adrenocorticotrophic hormone (...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1998.sp004151

    authors: Almeida SA,Anselmo-Franci JA,Rosa e Silva AA,Carvalho TL

    更新日期:1998-09-01 00:00:00

  • Systemic oxidative-nitrosative-inflammatory stress during acute exercise in hypoxia; implications for microvascular oxygenation and aerobic capacity.

    abstract::Exercise performance in hypoxia may be limited by a critical reduction in cerebral and skeletal tissue oxygenation, although the underlying mechanisms remain unclear. We examined whether increased systemic free radical accumulation during hypoxia would be associated with elevated microvascular deoxygenation and reduce...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/expphysiol.2014.081265

    authors: Woodside JD,Gutowski M,Fall L,James PE,McEneny J,Young IS,Ogoh S,Bailey DM

    更新日期:2014-12-01 00:00:00

  • Long non-coding RNA PRRT3-AS1 silencing inhibits prostate cancer cell proliferation and promotes apoptosis and autophagy.

    abstract:NEW FINDINGS:What is the central question of this study? What is the role of lncRNA PRRT3-AS1 in the regulation of peroxisome proliferator-activated receptor γ (PPARγ) gene-mediated mechanistic target of rapamycin (mTOR) signalling pathway in proliferation, apoptosis and autophagy of prostate cancer cells? What is the ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088011

    authors: Fan L,Li H,Wang W

    更新日期:2020-05-01 00:00:00

  • Cardiac parasympathetic outflow during dynamic exercise in humans estimated from power spectral analysis of P-P interval variability.

    abstract:NEW FINDINGS:What is the central question of this study? Should we use the high-frequency (HF) component of P-P interval as an index of cardiac parasympathetic nerve activity during moderate exercise? What is the main finding and its importance? The HF component of P-P interval variability remained even at a heart rate...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP085420

    authors: Takahashi M,Nakamoto T,Matsukawa K,Ishii K,Watanabe T,Sekikawa K,Hamada H

    更新日期:2016-03-01 00:00:00

  • White blood cell concentrations during lower body negative pressure and blood loss in humans.

    abstract:NEW FINDINGS:What is the central question of this study? Is lower body negative pressure a useful surrogate to study white blood cell responses to haemorrhage in humans? What is the main finding and its importance? We found that lower body negative pressure appears to be a useful surrogate to study the early white bloo...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/EP085952

    authors: van Helmond N,Johnson BD,Curry TB,Cap AP,Convertino VA,Joyner MJ

    更新日期:2016-10-01 00:00:00

  • Potential roles of ATP and local neurons in the monitoring of blood O2 content by rat aortic bodies.

    abstract::Aortic bodies are arterial chemoreceptors presumed to monitor blood O2 content by unknown mechanisms, in contrast to their well-studied carotid body counterparts, which monitor PO2 and /pH. We recently showed that rat aortic body chemoreceptors (type I cells), located at the left vagus-recurrent laryngeal nerve bifurc...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2013.075408

    authors: Piskuric NA,Zhang M,Vollmer C,Nurse CA

    更新日期:2014-01-01 00:00:00

  • Effect of feeding on the diurnal rhythm of plasma cortisol and adrenocorticotrophic hormone concentrations in the pregnant ewe and sheep fetus.

    abstract::The effects of two different feeding regimes on the 24 h profiles of maternal and fetal plasma cortisol and adrenocorticotrophic hormone (ACTH) concentrations were studied in eight pregnant ewes between 123 and 144 days of gestation. Once daily-fed ewes (n = 4) received 1 kg of lucerne-chaff at 11.00 h, and multi-fed ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1991.sp003488

    authors: Simonetta G,Walker DW,McMillen IC

    更新日期:1991-03-01 00:00:00

  • Age-related electrophysiological changes in mouse taste receptor cells.

    abstract:NEW FINDINGS:What is the central question of this study? Loss of taste or inability to distinguish between different tastes progresses with age. The purpose was to evaluate the age-dependent changes in taste by studying the electrophysiological properties of taste receptor cells. What is the main finding and its import...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP089104

    authors: Takeuchi K,Yoshii K,Ohtubo Y

    更新日期:2020-11-10 00:00:00

  • Resonant frequency at the wrist in hypermobile women.

    abstract::The resonant frequency of the wrist has been measured in sixteen hypermobile and nine normal women at ten levels of peak torque. At the lowest level the results were identical but at the others the hypermobile values were lower. Damping and thixotropy appeared to be normal. In another group the grip strength of hyperm...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1991.sp003493

    authors: Walsh EG,Lambert M,Wright GW,Powers N,Nuki G

    更新日期:1991-03-01 00:00:00

  • Interaction between genioglossus and diaphragm responses to transcranial magnetic stimulation in awake humans.

    abstract:UNLABELLED:The modulation of activity of the upper airway dilator and respiratory muscles plays a key role in the regulation of ventilation, but little is known about the link between their neuromuscular activation processes in vivo. This study investigated genioglossus and diaphragm responses to transcranial magnetic ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2007.037572

    authors: Wang W,Similowski T,Sériès F

    更新日期:2007-07-01 00:00:00

  • Is there something fishy about the regulation of the ryanodine receptor in the fish heart?

    abstract:NEW FINDINGS:What is the topic of this review? Excitation-contraction coupling in fish hearts is maintained over a range of temperatures that would be cardioplegic to most mammals. Here, we review what is known about the fish cardiac ryanodine receptor, and consider how it may be regulated in a different manner from th...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/EP085136

    authors: Shiels HA,Sitsapesan R

    更新日期:2015-12-01 00:00:00

  • Low-frequency power of heart rate variability is not a measure of cardiac sympathetic tone but may be a measure of modulation of cardiac autonomic outflows by baroreflexes.

    abstract::Power spectral analysis of heart rate variability has often been used to assess cardiac autonomic function; however, the relationship of low-frequency (LF) power of heart rate variability to cardiac sympathetic tone has been unclear. With or without adjustment for high-frequency (HF) power, total power or respiration,...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/expphysiol.2010.056259

    authors: Goldstein DS,Bentho O,Park MY,Sharabi Y

    更新日期:2011-12-01 00:00:00

  • Micropuncture and cannulation studies of fluid composition and transport in the ductuli efferentes testis of the rat: comparisons with the homologous metanephric proximal tubule.

    abstract::Luminal fluids were collected in vivo by micropuncture and cannulation from the rete testis, efferent ducts and ductus epididymidis of the rat to determine the composition of efferent duct fluids and the rates of reabsorption of water and solutes by the efferent ducts. The concentration of spermatozoa increased by a f...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1994.sp003817

    authors: Clulow J,Jones RC,Hansen LA

    更新日期:1994-11-01 00:00:00

  • Influence of hyperoxia on muscle metabolic responses and the power-duration relationship during severe-intensity exercise in humans: a 31P magnetic resonance spectroscopy study.

    abstract::Severe-intensity constant-work-rate exercise results in the attainment of maximal oxygen uptake, but the muscle metabolic milieu at the limit of tolerance (T(lim)) for such exercise remains to be elucidated. We hypothesized that T(lim) during severe-intensity exercise would be associated with the attainment of consist...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/expphysiol.2009.050500

    authors: Vanhatalo A,Fulford J,DiMenna FJ,Jones AM

    更新日期:2010-04-01 00:00:00

  • New insights into central cardiovascular control during exercise in humans: a central command update.

    abstract::The autonomic adjustments to exercise are mediated by central signals from the higher brain (central command) and by a peripheral reflex arising from working skeletal muscle (exercise pressor reflex), with further modulation provided by the arterial baroreflex. Although it is clear that central command, the exercise p...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/expphysiol.2005.032037

    authors: Williamson JW,Fadel PJ,Mitchell JH

    更新日期:2006-01-01 00:00:00

  • Cardiopulmonary and arterial baroreceptor unloading during passive hyperthermia does not contribute to hyperthermia-induced hyperventilation.

    abstract:NEW FINDINGS:What is the central question of this study? Does baroreceptor unloading during passive hyperthermia contribute to increases in ventilation and decreases in end-tidal carbon dioxide during that exposure? What is the main finding and its importance? Hyperthermic hyperventilation is not mitigated by expanding...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP085259

    authors: Lucas RA,Pearson J,Schlader ZJ,Crandall CG

    更新日期:2015-11-01 00:00:00

  • Changes in tension of slow motor units in rat medial gastrocnemius during constant-rate stimulation at different frequencies.

    abstract::Changes in tension of slow motor units in the medial gastrocnemius muscle of the rat were measured and compared in three fatigue tests, each of 4 min duration. In one of these tests motor unit tetani were evoked by repeated trains of stimuli of the standard frequency, 40 Hz, while in other tests frequencies of 20 and ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1995.sp003907

    authors: Celichowski J,Grottel K

    更新日期:1995-11-01 00:00:00

  • The expression of NFATc1 in adult rat skeletal muscle fibres.

    abstract::Although numerous studies have recently implicated the calcineurin-nuclear factor of activated T-cells (Cn-NFAT) signalling pathway in the regulation of activity-dependent fibre type switching in adult mammalian skeletal muscles, little is known about the endogenous expression of NFAT proteins in the various fibre typ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2007.040485

    authors: Mutungi G

    更新日期:2008-03-01 00:00:00

  • Foramen ovale blood flow and cardiac function after main pulmonary artery occlusion in fetal sheep.

    abstract:NEW FINDINGS:What is the central question of this study? At near-term gestation, foramen ovale blood flow accounts for a significant proportion of fetal left ventricular output. Can the foramen ovale increase its volume blood flow when right ventricular afterload is increased by main pulmonary artery occlusion? What is...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP087423

    authors: Lantto J,Erkinaro T,Haapsamo M,Huhta H,Voipio HM,Hohimer AR,Davis LE,Acharya G,Räsänen J

    更新日期:2019-02-01 00:00:00

  • Neuropeptidergic and autonomic control of the vasculature of the rat knee joint revealed by laser Doppler perfusion imaging.

    abstract::An imaging technique (laser Doppler perfusion imaging, LDI), based on measurement of backscattered Doppler-broadened laser radiation, was used to provide two-dimensional images of perfusion in the exposed rat knee joint capsule. In control animals, frequency-dependent constriction of knee joint blood vessels occurred ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1995.sp003851

    authors: Karimian SM,McDougall JJ,Ferrell WR

    更新日期:1995-05-01 00:00:00

  • The effect of H-wave therapy upon conduction in the human superficial radial nerve in vivo.

    abstract::This study was designed to investigate the putative neurophysiological effects of so-called 'H-wave therapy' (HWT), a form of electrical stimulation, upon conduction latencies in the human superficial radial nerve. Antidromic compound action potentials were recorded from the superficial radial nerve in the dominant fo...

    journal_title:Experimental physiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1113/expphysiol.1996.sp003979

    authors: McDowell BC,Lowe AS,Walsh DM,Baxter GD,Allen JM

    更新日期:1996-09-01 00:00:00

  • Ca(2+)-activated Cl- and K+ channels and their modulation by endothelin-1 in rat pulmonary arterial smooth muscle cells.

    abstract::Using the patch-clamp recording technique, we observed that endothelin-1 (ET-1; 0.8-16 nM) enhanced a voltage-activated outward current (Iout) and induced periodic oscillations of inward current in smooth muscle cells isolated from small pulmonary arteries (200-400 microns in diameter). Anion substitution experiments ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1995.sp003889

    authors: Salter KJ,Turner JL,Albarwani S,Clapp LH,Kozlowski RZ

    更新日期:1995-09-01 00:00:00