Abstract:
:The intrinsic proton buffering power (beta 1) of individual rat hippocampal and neocortical neurones maintained in culture has been investigated using the fluorescent dye 2', 7'-bis(carboxymethyl)-5, 6-(carboxyfluorescein) (BCECF). The steady-state intracellular pH (pH1) was estimated to be 7.03 +/- 0.04 (n = 22) in Hepes-buffered media and beta 1 estimated from the addition and removal of weak bases was ca 10 mM (pH unit)-1 at pH1 values near to 7. Estimates of beta 1 made from butyric acid challenges were inconsistent with estimates made at the same pH1, using NH4Cl withdrawal. However, estimating beta 1 with butyrate in the presence of the monocarboxylate ion transport inhibitor alpha-cyano-hydroxy-cinnamate (CHC) yielded beta 1 values commensurate with those measured using NH4Cl. Application of CHC alone lead to a rapid fall in pH1, suggesting a significant contribution of the monocarboxylate transporter to pH1 regulation. beta 1 was also estimated from a step increase in extracellular P(CO2). This yielded a value of 11 mM at an average pH1 of 7.1, which is similar to that of the other estimates reported here. beta 1 was found to increase with decreasing pH1: each unit drop in pH1 increased buffering power by about 60%. Blockade of pH1 regulation did not significantly affect estimates of beta 1. The change in buffering power with pH could be closely modelled from the known concentrations of free amino acids and organic phosphates.
journal_name
Exp Physioljournal_title
Experimental physiologyauthors
Amos BJ,Richards CDdoi
10.1113/expphysiol.1996.sp003930subject
Has Abstractpub_date
1996-03-01 00:00:00pages
261-71issue
2eissn
0958-0670issn
1469-445Xjournal_volume
81pub_type
杂志文章abstract::To probe the mechanisms by which respiratory syncytial virus (RSV) infection in early life forms an important risk factor for the development of chronic asthma, an airway hyper-responsiveness (AHR) animal model of guinea-pigs with persistent RSV infection was established by intranasal instillation of 2 x 10(5) plaque-...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2008.043521
更新日期:2008-12-01 00:00:00
abstract::This study examined the effect of pre-exercise carbohydrate (CHO) ingestion on pyruvate dehydrogenase complex (PDC) activation, acetyl group availability and substrate level phosphorylation (glycogenolysis and phosphocreatine (PCr) hydrolysis) in human skeletal muscle during the transition from rest to steady-state ex...
journal_title:Experimental physiology
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::Single cells from the rabbit pulmonary artery were isolated using a new and convenient procedure. Strips of muscle were incubated overnight in papain at 6 degrees C and dispersed the following morning after warming the tissue for 10 min. This method consistently produced a high yield of relaxed cells, which reversibly...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1991.sp003535
更新日期:1991-09-01 00:00:00
abstract::Vasopressin and oxytocin release from the neural lobe, and the vasopressin and oxytocin mRNA contents of the supraoptic and paraventricular nuclei are increased by hypertonicity of the extracellular fluid. The factors regulating these parameters can be conveniently studied in perifused explants of the hypothalamo-neur...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1111/j.1469-445x.2000.tb00021.x
更新日期:2000-03-01 00:00:00
abstract::Endothelium-dependent relaxation in response to histamine and ACh has been studied on precontracted aortic rings from control and salt-loaded Sprague-Dawley rats. Both ACh and histamine caused relaxation of the noradrenaline-induced precontraction only in the presence of the endothelium. The relaxation response to ACh...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1991.sp003475
更新日期:1991-01-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Regulation of cardiac function is typically achieved by changes in heart rate (HR) and cardiac shortening velocity (strain rate; SR), but their interdependence in vivo remains poorly understood. What is the main finding and its importance? Using resistance exerci...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP085081
更新日期:2015-04-20 00:00:00
abstract:NEW FINDINGS:What is the topic of this review? The manuscript collectively combines the experimental observations from >100 publications focusing on the regulation of cerebral blood flow and metabolism during exercise from 1945 to the present day. What advances does it highlight? This article highlights the importance ...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP086249
更新日期:2017-11-01 00:00:00
abstract::In humans, skeletal muscle blood flow is regulated by an interaction between several locally formed vasodilators, including NO and prostaglandins. In plasma, ATP is a potent vasodilator that stimulates the formation of NO and prostaglandins and, very importantly, can offset local sympathetic vasoconstriction. Adenosin...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/expphysiol.2014.081620
更新日期:2014-12-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Glucose is the dominant energy source for the brain. However, little is known about how glucose metabolism impacts the coordination of network activity in the brain in healthy adults. What is the main finding and its importance? We demonstrate that both alpha osc...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP089211
更新日期:2021-01-28 00:00:00
abstract::Using the patch-clamp recording technique, we observed that endothelin-1 (ET-1; 0.8-16 nM) enhanced a voltage-activated outward current (Iout) and induced periodic oscillations of inward current in smooth muscle cells isolated from small pulmonary arteries (200-400 microns in diameter). Anion substitution experiments ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1995.sp003889
更新日期:1995-09-01 00:00:00
abstract::The role of endothelium in the decreased contraction to phenylephrine in aorta of pregnant Wistar rats has been studied. Contractions in response to phenylephrine were significantly decreased in aortic rings from pregnant rats compared to those from non-pregnant rats. Pretreatment of the rings with indomethacin did no...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1993.sp003694
更新日期:1993-05-01 00:00:00
abstract::Plasma 2H accumulation was measured in six male volunteers after ingestion of drinks containing trace amounts of 2H2O. Subjects fasted overnight and remained seated at rest or exercised on a cycle ergometer at 42, 61 or 80% of their maximum oxygen uptake (VO2, max). The rate of plasma 2H accumulation was faster at res...
journal_title:Experimental physiology
pub_type: 临床试验,杂志文章
doi:10.1113/expphysiol.1990.sp003419
更新日期:1990-05-01 00:00:00
abstract::A method is described that enables the cell membrane of isolated rat ventricular myocytes to be permeabilized and resealed while maintaining cell viability. Streptolysin O, a cholesterol-binding cytolysin, was used to form pores in the surface membrane; subsequent incubation with 5% fetal bovine serum was used to reve...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1998.sp004114
更新日期:1998-05-01 00:00:00
abstract::The aim of the present work was to investigate the role of the serotoninergic system in the control of sodium appetite of hypothyroid rats (HTR) by administering drugs that affect the serotoninergic activity, and to compare the same homeostatic behaviour in euthyroid rats (ETR) also given these drugs. Fenfluramine (FE...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/eph8802457
更新日期:2003-03-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Impairment and subsequent improvement in cerebral oxygenation during acute and prolonged exposure to high altitude affect exercise performance. This study innovates by investigating the effect of acute and prolonged high-altitude exposure on cerebral haemodynamic...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP088354
更新日期:2021-01-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Pregnancy requires a robust plasma volume expansion driven by renal sodium retention. In the late-pregnant kidney, the aldosterone-responsive epithelial Na(+) channel is increased, whereas the sodium-chloride cotransporter is decreased. Pendrin has been shown to ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP085396
更新日期:2015-10-01 00:00:00
abstract::5-[5-(2-Nitrophenyl) furfuryliodine]-1,3-diphenyl-2-thiobarbituric acid (UCF-101) is a protease inhibitor which was reported to protect against ischaemic heart damage and apoptosis. This study evaluated the impact of UCF-101 on steptozotocin (STZ)-induced diabetic cardiomyocyte dysfunction. Adult FVB mice were made di...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2009.049189
更新日期:2009-09-01 00:00:00
abstract::A stress protocol--6 h of daily immobilization--was applied throughout male rat sexual development. Immobilization caused a small reduction in food intake and body weight gain whereas pair-fed animals had a marginal decrease only in body weight gain. Stress, confirmed by increased plasma adrenocorticotrophic hormone (...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1998.sp004151
更新日期:1998-09-01 00:00:00
abstract::This study was undertaken to determine the reflex cardiovascular and respiratory responses to discrete stimulation of pulmonary arterial baroreceptors using a preparation in which secondary modulation of responses from other reflexes was prevented. Dogs were anaesthetised with -chloralose, artificially ventilated, the...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? What are the molecular underpinnings of seasonal metabolic plasticity during spring and autumn migrations in songbirds? What is the main finding and its importance? We report differences in mRNA levels of genes involved in the regulation of glucose and fat metabo...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087974
更新日期:2019-12-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Is there sexual dimorphism in the occurrence of hepatic endoplasmic reticulum stress? What is the main finding and its importance? The transition from prepubertal to the adult age is associated with an increase in the unfolded protein response markers in the live...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087518
更新日期:2019-05-01 00:00:00
abstract::Latex beads coated with secretory immunoglobulin (IgA) instilled into mouse intestinal loops are taken up by M cells present in Peyer's patch follicle-associated epithelial tissue. Bead adsorption and uptake is greater at the edge compared with the apex of follicle domes. Coating beads with bovine serum albumin (BSA) ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.1992.sp003662
更新日期:1992-11-01 00:00:00
abstract::Moderate-intensity endurance exercise training improves vascular endothelial vasomotor function; however, the impact of high-intensity exercise training has been equivocal. Thus, the effect of the physiological stress of the exercise remains poorly understood. Furthermore, enhanced vascular repair mediated by circulat...
journal_title:Experimental physiology
pub_type: 临床试验,杂志文章
doi:10.1113/expphysiol.2011.062836
更新日期:2012-03-01 00:00:00
abstract::Exposure to whole-body vibration is implicated as one of the occupational risk factors for lower back disorders; however, its influence on the lumbar muscle physiology is still poorly understood. The objective of this study was to investigate the effects of backrest support and hand grip contractions on lumbar muscle ...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2006.033696
更新日期:2006-09-01 00:00:00
abstract:NEW FINDINGS:What is the topic of this review? The sympathetic control of renal sodium tubular reabsorption is dependent on activation of the intrarenal renin-angiotensin system and activation of the angiotensin II type 1 (AT1 ) receptor by angiotensin II. What advances does it highlight? Despite the fact that the inte...
journal_title:Experimental physiology
pub_type: 杂志文章,评审
doi:10.1113/EP085075
更新日期:2015-04-20 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Clinical studies suggest that obesity 'protects' against osteoporosis. However, these studies used only bone densitometry and assessed only one bone site, which is insufficient to enable conclusions to be drawn about the response of the whole skeleton. Furthermor...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/expphysiol.2014.082511
更新日期:2015-01-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Could different hormonally active substances, including oestrogen receptor (ER) agonists, protect against oxidative brain damage and memory impairment induced by a single epileptic seizure in rats? If so, which signalling mechanisms are involved in their anti-inf...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087986
更新日期:2019-12-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? Our previous study demonstrates that elevated orexin 2 receptor (OX2R) activity within the rostral ventrolateral medulla (RVLM) contributes to hypertension in spontaneously hypertensive rats (SHRs), and a lower OX2R protein level was detected in their RVLM. The p...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP085016
更新日期:2015-09-01 00:00:00
abstract::What is the central question of this study? Neurocognitive functions can be enhanced by acute aerobic exercise, which could be associated with changes in serum brain-derived neurotrophic factor (BDNF) concentrations. We aimed to explore acute exercise-induced changes in BDNF concentrations, neuropsychological and neur...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP085682
更新日期:2016-07-01 00:00:00
abstract:NEW FINDINGS:What is the central question of this study? What is the role of miR-143-3p during human dental pulp stem cell (hDPSC) differentiation. What is the main finding and its importance? miR-143-3p negatively regulates receptor activator of nuclear factor-κB (RANK). RANK ligand (RANKL) binds to RANK and stimulate...
journal_title:Experimental physiology
pub_type: 杂志文章
doi:10.1113/EP087992
更新日期:2020-05-01 00:00:00