[Usefulness of AgNOR technique in the interpretation of serous effusions].

Abstract:

BACKGROUND:AgNOR technique detects, using silver salts, argyrophylic proteins of the nucleolar organizer region (NOR). The number and size of NOR reflect cell activity, proliferation and transformation and may help to differentiate benign from malignant cells. AIM:To assess the value of AgNOR assay to differentiate reactive mesothelial cells from malignant cells in serous effusions. MATERIAL AND METHODS:Thirty one fluids obtained from 16 pleural, 14 peritoneal and one pericardial effusion, were studied. The fluids were processed with Giemsa and Papanicolau stains and with the AgNOR technique. The number of AgNOR dots were counted (only when it was possible to distinguish each individual dot) and the mean value per nucleus was calculated for each smear. RESULTS:Mesothelial cells had a mean of 4.88 +/- 1.5 dots compared with 13.78 +/- 3.88 dots in the malignant cells (p < 0.001). CONCLUSIONS:AgNOR assay can be useful for the differentiation of benign and malignant cells in serous effusions.

journal_name

Rev Med Chil

journal_title

Revista medica de Chile

authors

Rocher AE,Blanco AM,Palaoro LA

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

963-8

issue

9

eissn

0034-9887

issn

0717-6163

journal_volume

128

pub_type

杂志文章
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    authors: Boza C,Dagnino B,Garrido AM,Llanos O

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  • [Current life expectancy in Chile].

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    authors: Medina Lois E

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  • [Clinical Practice Guidelines for Smoking Cessation].

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    pub_type: 临床试验,杂志文章,随机对照试验

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    authors: Solis F,Castillo B,Mardones G

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  • [Medical coverage of the disease that causes death and place of death in the sixth region of Chile from 1990 to 2003].

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    authors: Sierralta A,Jeria ME,Figueroa G,Pinto J,Araya JC,San Juan J,Grinbergs J,Valenzuela E

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