Abstract:
:Based on their typical pattern of membrane currents, four populations of glial cells could be identified in thin brain slices of the postnatal hippocampus. In the present study, we applied the patch-clamp technique to glial cells in the hippocampal CA1 region, which are characterized by a complex pattern of different Na+ and K+ currents ("complex" cells). These cells were identified as non-neuronal cells, most likely astrocytes, by their glutamine synthetase immunoreactivity. Two types of glial Ca2+ currents could be identified that differed in their kinetics and pharmacological properties. A low-voltage activated (LVA), fast inactivating component was activated at membrane potentials positive to -60 mV and reached maximum current amplitudes at about -20 mV. This current was sensitive to amiloride and thus displayed properties of neuronal LVA currents. The threshold potential of the second Ca2+ current component was at about -40 mV, and peak currents were observed at 0 mV. In contrast to the LVA component, the inactivation of these high-voltage activated (HVA) currents slowed down with increasing depolarizations. This current was sensitive to low concentrations of Cd2+ but was not affected by amiloride. A small fraction of the HVA currents was sensitive to nifedipine, and omega-conotoxin GVIA (omega-CgTx) was also found to reduce the glial HVA component. The study provides electrophysiological and pharmacological characterization of different types of Ca2+ currents in gray matter glial cells in situ.
journal_name
Gliajournal_title
Gliaauthors
Akopian G,Kressin K,Derouiche A,Steinhäuser Cdoi
10.1002/(SICI)1098-1136(199607)17:3<181::AID-GLIA1subject
Has Abstractpub_date
1996-07-01 00:00:00pages
181-94issue
3eissn
0894-1491issn
1098-1136pii
10.1002/(SICI)1098-1136(199607)17:3<181::AID-GLIA1journal_volume
17pub_type
杂志文章相关文献
GLIA文献大全abstract::During axonal ensheathment, noncompact myelin channels formed at lateral edges of the myelinating process become arranged into tight paranodal spirals that resemble loops when cut in cross section. These adhere to the axon, concentrating voltage-dependent sodium channels at nodes of Ranvier and patterning the surround...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23809
更新日期:2020-09-01 00:00:00
abstract::Gliogenesis in the mammalian CNS continues after birth, with astrocytes being generated well into the first two postnatal weeks. In this study, we have isolated an A2B5(+) astrocyte precursor (APC) from the postnatal rat forebrain, which is capable of differentiating into mature astrocytes in serum-free medium without...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20788
更新日期:2009-04-15 00:00:00
abstract::Extracellular purines are signaling molecules in the neurogenic niches of the brain and spinal cord, where they activate cell surface purinoceptors at embryonic neural stem cells (NSCs) and adult neural progenitor cells (NPCs). Although mRNA and protein are expressed at NSCs/NPCs for almost all subtypes of the nucleot...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.23056
更新日期:2017-02-01 00:00:00
abstract::We first classified 12 malignant glioma cell lines into three different groups (types 1-3) with respect to major histocompatibility complex (MHC) class II expression and analyzed each group based on the different expression status of the class II transactivator (CIITA) gene. Glioma type 1 (2 of 12) showed constitutive...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10343
更新日期:2004-03-01 00:00:00
abstract::Neurosteroids, such as progesterone, influence central nervous system development and function by regulating a broad spectrum of physiological processes. Here, we investigated membrane-initiated actions of progesterone in the retina and identified the membrane-associated progesterone receptor component 1 (PGRMC1). We ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20523
更新日期:2007-08-01 00:00:00
abstract::Our knowledge of astroglia and their physiological and pathophysiological role(s) in the central nervous system (CNS) has grown during the past decade, revealing a complex picture. It is becoming increasingly clear that glia play a significant role in the homeostasis and function of the CNS and that neurons should no ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20213
更新日期:2005-06-01 00:00:00
abstract::NG2 is a type 1 integral membrane glycoprotein encoded by the Cspg4 gene. It is expressed on glial progenitor cells known as NG2 glial cells or oligodendrocyte precursor cells that exist widely throughout the developing and mature central nervous system and vascular mural cells but not on mature oligodendrocytes, astr...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23521
更新日期:2018-12-01 00:00:00
abstract::Under pathological conditions, activated microglia play paradoxical roles and could have neurotoxic or neuroprotective effects. However, the signal determining how activated microglia affects the fate of neuronal cells remains largely unknown. Here we demonstrate that DNAX-activating protein of 12 kDa (DAP12), a trans...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22802
更新日期:2015-06-01 00:00:00
abstract::Synemin, a very unique type VI intermediate filament (IF) protein, exhibits alternative splice variants termed alpha and beta. Unlike other IF proteins, synemin binds to actin-associated proteins, including alpha-actinin, vinculin, and alpha-dystrobrevin. Our previous work has demonstrated the presence of synemin in d...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20158
更新日期:2005-04-15 00:00:00
abstract::Galactosylceramide (GalC) is the major sphingolipid of the myelin membrane. Mice lacking GalC due to ceramide galactosyltransferase (CGT) deficiency form unstable and functionally affected myelin and exhibit a progressive demyelination, accompanied by severe motor coordination deficits. In addition to oligodendrocytes...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20230
更新日期:2005-11-15 00:00:00
abstract::Astrocytes extend thin lamellate processes in the neuropil, in particular around synapses, where they can modulate synaptic function or mediate glial-neuronal communication. Previous studies have shown that these lamellate perisynaptic processes change their shape in response to neuronal activity, but the underlying m...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1120
更新日期:2001-12-01 00:00:00
abstract::In multiple sclerosis (MS), one strategy to reduce disability is enhancement of endogenous repair by remyelinating oligodendrocytes derived from oligodendrocyte progenitor cells (OP). An important prerequisite is determining the abundance of OP relative to oligodendrocytes in normal human central nervous system (CNS),...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21018
更新日期:2010-09-01 00:00:00
abstract::Microglia survey the brain environment by sensing several types of diffusible molecules, among which extracellular nucleotides released/leaked from damaged cells have central roles. Microglia sense ATP or other nucleotides by multiple P2 receptors, after which they change into several different phenotypes. However, so...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22517
更新日期:2013-08-01 00:00:00
abstract::Oligodendrocytes, the myelinating cells of the CNS, are derived postnatally from oligodendrocyte precursors (OPs) of the subventricular zone (SVZ). However, the mechanisms that regulate their generation from SVZ neural stem cells (NSC) are poorly understood. Here, we have examined the role of glycogen synthase kinase ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22641
更新日期:2014-05-01 00:00:00
abstract::Sulfated glycosaminoglycans (S-GAGs) were isolated from the pericellular (P), intracellular (I), and extracellular (E) compartments of astrocytes cultures from lateral (L) and medial (M) sectors of embryonic mouse midbrain; these sectors differ in their ability to support neurite growth (L, permissive, M, non-permissi...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(SICI)1098-1136(199608)17:4<339::AID-GLIA8
更新日期:1996-08-01 00:00:00
abstract::Injury to the mammalian spinal cord is accompanied by a delayed, secondary wave of oligodendrocyte apoptosis that arises several days after the initial injury. A strong candidate to support oligodendrocyte survival after spinal cord injury is the pleiotropic cytokine, leukemia inhibitory factor (LIF). In vitro, LIF po...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20177
更新日期:2005-07-01 00:00:00
abstract::Microglia show a rich repertoire of activation patterns regulated by a complex ensemble of surface ion channels, receptors, and transporters. We and others have investigated whether microglia vary their K+ channel expression as a means to achieve functional diversity. However, most of the prior studies were conducted ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23457
更新日期:2018-09-01 00:00:00
abstract::This report describes the development of a new panel of monoclonal antibodies produced following immunization of mice with cultured rat microglial cells. Using these new reagents and previously defined antibodies that bind to microglia or macrophages, the responses of parenchymal microglia, perivascular "microglial" c...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440070108
更新日期:1993-01-01 00:00:00
abstract::Microglial cells have a double life as the immune cells of the brain in times of stress but have also specific physiological functions in homeostatic conditions. In pathological contexts, microglia undergo a phenotypic switch called "reaction" that promotes the initiation and the propagation of neuro-inflammation. Rea...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23295
更新日期:2018-05-01 00:00:00
abstract::Neurons in organotypic hippocampal slice cultures (OHSCs) are known to preserve morphological and physiological features of the in vivo situation; however, little is known about the properties of microglial cells under these in vitro conditions. In this study, we addressed the question whether microglial cells in OHSC...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199612)18:4<319::aid-glia6
更新日期:1996-12-01 00:00:00
abstract::Whole-cell membrane currents and imaging of intracellular calcium concentrations ([Ca2+]i) were used to investigate the role of calcium in a response to serotonin of C6 glioma cells. Activation of a high-affinity serotonin receptor induced a transient rise in calcium concentration in these cells and activated a predom...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440060206
更新日期:1992-01-01 00:00:00
abstract::We have previously reported that neonatal rat oligodendrocytes (OLGs) express and secrete neuregulins (NRGs) (Raabe et al., 1997). This laboratory has also shown that NRGs stimulate the differentiation of neonatal rat OLGs and that these cells express the erbB receptors for NRGs (Raabe et al., 1997). In this study, we...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Nogo-A is a potent glial-derived inhibitor of axon growth in the injured CNS and acts as a negative regulator of developmental angiogenesis by inhibiting vascular endothelial cell migration. However, its function in pathological angiogenesis has never been studied after ischemic injury in the CNS. Using the mouse mode...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23462
更新日期:2018-10-01 00:00:00
abstract::Astrocytic proliferation and hypertrophy (astrogliosis) are associated with neuronal injury. However, neither the temporal nor the spatial relationship between astrocytes and injured neurons is clear, especially in neurodegenerative diseases. We investigated these questions in a mouse amyotrophic lateral sclerosis (AL...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Mitogenic growth factors play an important role in the initial stages of oligodendrocyte development, but their roles in the process of myelination itself remain less well defined. In order to study directly the effects of different growth factors on myelination, we used a purified in vitro co-culture system with dors...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20480
更新日期:2007-04-01 00:00:00
abstract::Stellate ganglion neurons, important mediators of cardiopulmonary neurotransmission, are surrounded by satellite glial cells (SGCs), which are essential for the function, maintenance, and development of neurons. However, it remains unknown whether SGCs in adult sympathetic ganglia exhibit any functional diversity, and...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23965
更新日期:2021-01-12 00:00:00
abstract::Annexin II (AX II) is a member of the family of calcium-dependent actin- and phospholipid-binding proteins implicated in numerous intracellular functions such as signal transduction, membrane trafficking, and mRNA transport, as well as in the regulation of membrane/cytoskeleton contacts and extracellular functions. AX...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20529
更新日期:2007-08-01 00:00:00
abstract::We have shown previously that switching of the alpha v-associated beta1 and beta5 integrin subunits during differentiation of myelin-forming oligodendrocytes may regulate important aspects of cell behaviour such as migration (Milner et al., 1996: J Neurosci 16:7240-7252). In this study we have examined the development...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::There is increasing evidence that chemokines, specialized regulators of the peripheral immune system, are also involved in the physiology and pathology of the CNS. It is known that glial cells (astrocytes and microglia) express various chemokine receptors like CCR1, -3, -5, and CXCR4. We have investigated the possible...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10156
更新日期:2003-03-01 00:00:00
abstract::The detection of nucleic acids by the innate immune system is an essential host response during viral infection. In recent years, a number of immune sensors capable of recognizing cytosolic DNA have been identified and include the PYHIN family members AIM2, IFI16, and p204 as well as the enzyme, cGAS. Activation of th...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22786
更新日期:2015-05-01 00:00:00