Abstract：
:In this study subcellular localization of cyclin A in the human promyelocytic leukemia cell line HL-60 and the human erythroleukemic K-562 cell line was examined by immunocytochemical methods. Studies were based on light and electron microscope evaluations. Cyclin A at the level of light microscope was present in 48% of the cells in HL-60 cell line and in 40% of the cells in K-562 line. Streptavidin-gold method was used for localization of cyclin A at the ultrastructural level. There was expression of cyclin A in the nucleus and in the cytoplasm. In the nucleus gold particles were seen to be associated with the condensed chromatin of the both leukemia cell lines. In the cytoplasm cyclin A was concentrated at a low level and was associated with ribosomes. Controls of the leukemia cells incubated with normal mouse serum showed no labeling at the light and electron microscope level.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Grzanka A,Styczynski Jsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
112-5issue
2eissn
0028-2685issn
1338-4317journal_volume
48pub_type
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