Abstract：
:The aim of our work was to develop a fast, reliable and sensitive PCR method to detect K-ras mutations in various clinical samples. There is a need for an unimpeachable method for early diagnosis and/or screening of pancreatic cancer (PC). We optimized and subsequently analyzed four methods based on mutant-enriched PCR for the sensitivity, cost and time expense. Using the selected optimal method we examined codon 12 K- ras mutations in a study population of 59 patients with upper GIT malignancies. Reliability of the genotyping was confirmed by sequencing. By using the best of our modified mutant-enriched PCR methods we achieved sensitivity of 1:1 x 10(5). Further studies are necessary to determine the optimal biological material sampling in PC.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Ugorcáková J,Hlavatý T,Babál P,Janega P,Bukovská G,Halgasová N,Hlavatá A,Timko J,Duris Isubject
Has Abstractpub_date
2006-01-01 00:00:00pages
363-71issue
5eissn
0028-2685issn
1338-4317journal_volume
53pub_type
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