Abstract：
:This study was undertaken to examine the influence of etoposide and doxorubicin on the distribution of vimentin in cells of human leukemia cell line K-562 by using immunofluorescence microscopy. The cells were cultured with 5 different doses of etoposide: 0.02, 0.2, 2, 20, 200 microM/l and three doses of doxorubicin: 0.5, 5, 10 microM/l. Changes in vimentin filaments were dependent on concentration of drugs compared to untreated control cells. Cells treated with 20 microM/l, especially with 200 microM/l etoposide were much bigger from other cells exposed to lower doses of etoposide and control cells, and their number decreased. In most control cells vimentin was seen as a ring with the increased concentration on one pole of the cells. In 20 microM/l and 200 microM/l etoposide the cells showed rather a diffuse cytoplasmic staining pattern. Vimentin filaments were organized as a dense network in cytoplasm of these cells. Immunofluorescence studies on K-562 cells treated with doxorubicin showed that cells incubated with 5 microM/l doxorubicin have much diffuse staining pattern of vimentin with delicate reticular structure and with intense staining near one pool of the cells. Addition of 10 microM/l doxorubicin to cells resulted in increasing of fluorescence staining, which appeared in the cells as enough dense network with intense staining rather in the centre of the cell.
journal_name
Neoplasmajournal_title
Neoplasmaauthors
Grzanka Asubject
Has Abstractpub_date
2001-01-01 00:00:00pages
285-9issue
4eissn
0028-2685issn
1338-4317journal_volume
48pub_type
杂志文章相关文献
NEOPLASMA文献大全abstract：:The efficacy of cis-platinum in the treatment of recurrent advanced malignant melanoma is discussed and also the so far unpublished clinical data have been summed up. On account of the considerable differences among these data the clinical study of Stage II had been started which should contribute to the elucidation o...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Zinc finger protein 139(ZNF139), a member of zinc finger protein family, is a transcription factor. Our previous research showed ZNF139 was overexpressed in gastric cancer cells. The purpose of present study is to explore impact and mechanism of ZNF139 on metastasis by regulating invasive ability of gastric cancer cel...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2014_037
更新日期：2014-01-01 00:00:00
abstract：:NAT2 as phase II enzyme is involved in the detoxification/activation of various drugs, environmental substances and carcinogenic compounds. A genotyping approach has been used to investigate NAT2 genotype with putative relevance in lung cancer in population of 110 Slovak-Caucasians patients and 167 non-malignant indiv...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Laryngeal carcinoma is the second commonest head and neck carcinoma globally. MicroRNA-101 (miR-101) has been reported as a tumor suppressor in multiple malignancies including laryngeal squamous cell carcinoma (LSCC). However, the roles and molecular mechanisms of miR-101 in the development of LSCC have not been fully...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_180811N611
更新日期：2019-07-23 00:00:00
abstract：:Therapeutic effect of Cyclophosphamide (CPA) and radiosensitizer AK-2123 (AK) combination versus CPA alone in the same doses was investigated on transplanted LL carcinoma and B 16 melanoma. Antimetastatic efficacy of different doses of CPA and combined therapy was evaluated. Our data demonstrate that the effect of com...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is the second leading cause of cancer mortality worldwide. Although advances have made in treatment of HCC, the overall survival rate remains low and the molecular pathogenesis of HCC is still poorly understood. The purpose of this study was to explore the molecular pathogenesis of HCC. ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2017_516
更新日期：2017-01-01 00:00:00
abstract：:The paclitaxel represents first agent from novel class of antineoplastic drugs-taxoids. The clinical development of paclitaxel was initially hampered by hypersensitivity reactions. Current dosage regiments with premedication reduced the incidence of these side effects to less than 3%. The major dose-limiting adverse e...
journal_title：Neoplasma
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:In vivo immunogenicity and in vitro species-specific membrane antigens in tumor cells treated or untreated with glutaraldehyde (GA) were studied. Two different syngeneic Syrian hamster transplantable tumor cell lines (spontaneous liver cancer and SV40-induced sarcoma) not only lost immunogenicity after GA treatment bu...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:This study aimed to identify the oncogenes associated with lung cancer based on the mRNA and single nucleotide polymorphism (SNP) profile data. The mRNA expression profile data of GSE43458 (80 cancer and 30 normal samples) and SNP profile data of GSE33355 (61 pairs of lung cancer samples and control samples) were down...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2015_117
更新日期：2015-01-01 00:00:00
abstract：:To monitor the specific antitumor immunity to patients with malignant melanoma of the uveal tissue, use was made of the 20-hr modification of the leukocyte adherence inhibition (LAI) test. Simultaneously, changes in the proportionally of T (total and active) and B lymphocytes in peripheral blood were followed in the s...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Serological analysis of human membrane antigens have been performed using rabbit xenosera against membrane fraction obtained from different cells (B cell lines: RAJI, RAMOS, DAUDI, UHKT-2: T cell line: MOLT-3, as well as normal pooled lymphocytes). The direct cytotoxicity testing demonstrated that xenogeneic rabbit B ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:In our earlier report on circulating immune complexes (CIC) from sera of patients with Hodgkin's disease (HD), we demonstrated disease-associated increase in the intensity of a 40kD (pl 5.6) polypeptide in the CIC. In extension of these investigations, we now report that the 40kD moiety exists in CIC as a complex with...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Molecular targeted therapy based on tyrosine kinase inhibitors, directed at the epidermal growth factor receptor (EGFR) is one of novel options for management of NSCLC. Erlotinib is EGFR tyrosine kinase inhibitor used for treatment of the advanced NSCLC. This presented study is focused on comparison of erlotinib and c...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2013_017
更新日期：2013-01-01 00:00:00
abstract：:During the decade 1968-1977 a total of 1488 cases of neoplasms were registered in Slovakia in children aged 0-14 years giving an average annual age-specific rate of 120.7 per million. Tumors prevailed in boys with 131.2 in comparison with girls showing 109.7 incidence rate/10(6). The most common malignancies were leuk...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Human immediate early response 2 (IER2) has been implicated in tumor cell motility and metastasis; however, the underlying mechanisms in hepatocellular carcinoma (HCC) metastasis remain to be clarified. In this study, we demonstrate that dysregulation of IER2 was shown in HCC clinical samples, and IER2 expression resu...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2020_190818N781
更新日期：2020-05-01 00:00:00
abstract：:During the last 30 years the trends in cancer mortality in Europe significantly changed. In 1970 the male and female cancer mortality (all ages) was higher in the Western Europe than in the "socialist" Central and Eastern Europe. After 1970 in most Western countries decrease or no change of male premature (0-64 years)...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Considering the potency of circRNAs in manifesting neoplastic progression, we attempted to explore the feasibility of applying ciRS-7 for diagnosis and prognosis estimation of cervical cancer (CC). Here 352 CC patients, 204 cervical intraepithelial neoplasia (CIN) patients and 227 healthy controls were recruited. The ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2019_190415N334
更新日期：2020-03-01 00:00:00
abstract：:Regional lymph nodes in 80 cases of gastric and 70 cases of breast cancer have been examined and the reactive types have been correlated to the survival periods of the patients. In both groups of patients, a prognostically favorable sign was the detection of lymphocytic predominance combined with sinus histiocytosis, ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:It is generally believed that the existence of cancer stem cells (CSCs) is related to tumor recurrence and metastasis of hepatocellular carcinoma (HCC). Neuropilin1 (NRP1) is involved in numerous pathophysiological processes of tumor progression, however, whether NRP1 is involved in the regulation of liver CSCs and me...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2020_200914N982
更新日期：2020-12-23 00:00:00
abstract：:The effect of heterologous antimacrophage serum (AMS) and normal rabbit serum (NRS) on the immune reaction against Rous virus-induced sarcoma (RSL) was studied in rats. The growth of RSL sarcoma transplanted against the H-1 barrier in AMS-treated rats was more progressive than in the untreated or NRS-treated control g...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:The present study was carried out to investigate the synergistic apoptotic potential of arabinoxylan rice bran (MGN-3/Biobran) and curcumin (turmeric) on human multiple myeloma (MM) cell line U266 , in vitro. U266 cells were cultured with MGN-3 (50 or 100μg/ml) and curcumin (2.5-10μM) for 3 days. The effects of MGN-3 ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2011_02_118
更新日期：2011-01-01 00:00:00
abstract：:Immunohistochemistry (IE) and polymerase chain reaction (PCR) are tools enabling to find small number of tumor cells in lymph nodes (LNs) or peripheral blood. Perhaps these methods will allow early detection of cell dissemination and refine risk group within papillary thyroid cancer (PTC) that might benefit from more ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The isoquinoline peripheral benzodiazepine receptor ligand PK11195 increased drug (daunomycin)- and fluorochrome (calcein-AM) uptake and induced apoptosis detected by flow cytometry (FCM) technique, DNA electrophoretic analysis and poly(ADP-ribose) polymerase (PARP) cleavage in human multidrug-resistant myeloid leukem...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Cancer cells share numerous characteristics with pluripotent stem cells which develop normally, contributing to the tumor cell plasticity. So we investigated the effect of human embryonic germ cells (hEGCs) on SKOV3 cells. We examined the efficacy of hEGCs through coculturing SKOV3 with hEGCs, detecting tunel apoptosi...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2009_01_13
更新日期：2009-01-01 00:00:00
abstract：:HCC is one of the leading causes of death worldwide. Liver transplantation including living donor transplantation is the best available treatment. We have analyzed our experience with LDLT in patients with HCC and HCV in order to determine if alpha feto-protein (AFP) is a better predictor of recurrence than the tumor ...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_170315N184
更新日期：2018-03-14 00:00:00
abstract：:The cancer chemotherapeutic efficacy of 3,4-dihydroxybenzylamine (DHBA), a dopamine analog with reduced neurotoxic effects, was evaluated in strain A mice bearing transplantable Ehrlich's ascites carcinoma. The analog was administered intraperitoneally on day 1 post-transplantation at dose schedules of 50, 100 and 200...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Circular RNAs (circRNAs) are a class of newly-identified non-coding RNA molecules. CircRNAs are conserved across different species and display specific organization, sequence, and expression in disease. Moreover, circRNAs' closed ring structure, insensitivity to RNase, and stability are advantages over linear RNAs in ...
journal_title：Neoplasma
pub_type： 杂志文章,评审
doi：10.4149/neo_2017_301
更新日期：2017-01-01 00:00:00
abstract：:This study was supposed to investigate the correlation between the functional single nucleotide polymorphisms (SNPs) (rs2516839 and rs3737787) in USF1 gene and the efficacy and safety of paclitaxel-based chemotherapy and prognosis in the treatment of ovarian cancer (OC). In total 100 OC patients were selected and divi...
journal_title：Neoplasma
pub_type： 杂志文章
doi：10.4149/neo_2018_170322N205
更新日期：2018-01-01 00:00:00
abstract：:The effects of 9-beta-D-arabinofuranosyladenine (araA) on radiosensitivity, postirradiation changes in the cell cycle distribution and DNA synthesis in mouse L5178Y-S lymphoma cells are described. Increase in the araA concentration (from 25 to 200 mumol/l) was is correlated with reduction in clonogenic survival except...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Eucalyptol, carvacrol and thymol represent components of plant essential oils characterized by a wide range of biological effects toward microorganisms, fungi, insects, etc. However, till now only a few investigations have been carried out to study the effects of essential oils and their components on human cells cult...
journal_title：Neoplasma
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
