Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.


:clpC of Bacillus subtilis is part of an operon containing six genes. Northern blot analysis suggested that all genes are co-transcribed and encode stress-inducible proteins. Two promoters (PA and PB) were mapped upstream of the first gene. PA resembles promoters recognized by the vegetative RNA polymerase E sigma A. The other promoter (PB) was shown to be dependent on sigma B, the general stress sigma factor in B. subtilis, suggesting that clpC, a potential chaperone, is expressed in a sigma B-dependent manner. This is the first evidence that sigma B in B. subtilis is involved in controlling the expression of a gene whose counterpart, clpB, is subject to regulation by sigma 32 in Escherichia coli, indicating a new function of sigma B-dependent general stress proteins. PB deviated from the consensus sequence of sigma B promoters and was only slightly induced by starvation conditions. Nevertheless, strong induction by heat, ethanol, and salt stress occurred at the sigma B-dependent promoter, whereas the vegetative promoter was only weakly induced under these conditions. However, in a sigB mutant, the sigma A-like promoter became inducible by heat and ethanol stress, completely compensating for sigB deficiency. Only the downstream sigma A-like promoter was induced by certain stress conditions such as hydrogen peroxide or puromycin. These results suggest that novel stress-induction mechanisms are acting at a vegetative promoter. Involvement of additional elements in this mode of induction are discussed.


Mol Microbiol


Molecular microbiology


Krüger E,Msadek T,Hecker M




Has Abstract


1996-05-01 00:00:00












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    journal_title:Molecular microbiology

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    authors: Suzuki I,Kanesaki Y,Mikami K,Kanehisa M,Murata N

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  • Fibronectin-binding proteins of Staphylococcus aureus mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb/IIIa and IgG binding to the FcgammaRIIa receptor.

    abstract::Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. aureus resulting in aggregation and thrombus formation is an important step in the pathogenesis of IE. Here, we report that the fibrinogen/fibronectin-binding proteins FnBPA and FnBPB are major platelet-activati...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Fitzgerald JR,Loughman A,Keane F,Brennan M,Knobel M,Higgins J,Visai L,Speziale P,Cox D,Foster TJ

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  • To ∼P or Not to ∼P? Non-canonical activation by two-component response regulators.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Desai SK,Kenney LJ

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    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Volff JN,Altenbuchner J

    更新日期:1998-01-01 00:00:00

  • Structure and function of Listeria teichoic acids and their implications.

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    authors: Sumrall ET,Keller AP,Shen Y,Loessner MJ

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    authors: Smith RW,McAteer S,Masters M

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    authors: Marquis H,Hager EJ

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    authors: Webster C,Merryweather A,Brammar W

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    authors: Haselkorn R

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    pub_type: 杂志文章,评审


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Guo BP,Brown EL,Dorward DW,Rosenberg LC,Höök M

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Richardson AR,Stojiljkovic I

    更新日期:2001-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Huang HY,Tang HL,Chao HY,Yeh LS,Wang CC

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Camacho LR,Ensergueix D,Perez E,Gicquel B,Guilhot C

    更新日期:1999-10-01 00:00:00