Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.

Abstract:

:clpC of Bacillus subtilis is part of an operon containing six genes. Northern blot analysis suggested that all genes are co-transcribed and encode stress-inducible proteins. Two promoters (PA and PB) were mapped upstream of the first gene. PA resembles promoters recognized by the vegetative RNA polymerase E sigma A. The other promoter (PB) was shown to be dependent on sigma B, the general stress sigma factor in B. subtilis, suggesting that clpC, a potential chaperone, is expressed in a sigma B-dependent manner. This is the first evidence that sigma B in B. subtilis is involved in controlling the expression of a gene whose counterpart, clpB, is subject to regulation by sigma 32 in Escherichia coli, indicating a new function of sigma B-dependent general stress proteins. PB deviated from the consensus sequence of sigma B promoters and was only slightly induced by starvation conditions. Nevertheless, strong induction by heat, ethanol, and salt stress occurred at the sigma B-dependent promoter, whereas the vegetative promoter was only weakly induced under these conditions. However, in a sigB mutant, the sigma A-like promoter became inducible by heat and ethanol stress, completely compensating for sigB deficiency. Only the downstream sigma A-like promoter was induced by certain stress conditions such as hydrogen peroxide or puromycin. These results suggest that novel stress-induction mechanisms are acting at a vegetative promoter. Involvement of additional elements in this mode of induction are discussed.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Krüger E,Msadek T,Hecker M

doi

10.1111/j.1365-2958.1996.tb02511.x

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

713-23

issue

4

eissn

0950-382X

issn

1365-2958

journal_volume

20

pub_type

杂志文章
  • Cold-regulated genes under control of the cold sensor Hik33 in Synechocystis.

    abstract::A histidine kinase, Hik33, appears to sense decreases in temperature and to regulate the expression of certain cold-inducible genes in the cyanobacterium Synechocystis sp. PCC6803. To examine the role of Hik33 in the regulation of gene expression, we analysed a DeltaHik33 mutant using the DNA microarray technique. In ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02379.x

    authors: Suzuki I,Kanesaki Y,Mikami K,Kanehisa M,Murata N

    更新日期:2001-04-01 00:00:00

  • Fibronectin-binding proteins of Staphylococcus aureus mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb/IIIa and IgG binding to the FcgammaRIIa receptor.

    abstract::Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. aureus resulting in aggregation and thrombus formation is an important step in the pathogenesis of IE. Here, we report that the fibrinogen/fibronectin-binding proteins FnBPA and FnBPB are major platelet-activati...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2005.04922.x

    authors: Fitzgerald JR,Loughman A,Keane F,Brennan M,Knobel M,Higgins J,Visai L,Speziale P,Cox D,Foster TJ

    更新日期:2006-01-01 00:00:00

  • To ∼P or Not to ∼P? Non-canonical activation by two-component response regulators.

    abstract::Bacteria sense and respond to their environment through the use of two-component regulatory systems. The ability to adapt to a wide range of environmental stresses is directly related to the number of two-component systems an organism possesses. Recent advances in this area have identified numerous variations on the a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/mmi.13532

    authors: Desai SK,Kenney LJ

    更新日期:2017-01-01 00:00:00

  • Genetic instability of the Streptomyces chromosome.

    abstract::The Streptomyces wild-type chromosome is linear in all examples studied. The ends of the chromosome or telomeres consist of terminal inverted repeats of various sizes with proteins covalently bound to their 5' ends. The chromosome is very unstable and undergoes very large deletions spontaneously at rates higher than 0...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2958.1998.00652.x

    authors: Volff JN,Altenbuchner J

    更新日期:1998-01-01 00:00:00

  • Structure and function of Listeria teichoic acids and their implications.

    abstract::Teichoic acids (TAs) are the most abundant glycopolymers in the cell wall of Listeria, an opportunistic Gram-positive pathogen that causes severe foodborne infections. Two different structural classes of Listeria TA exist: the polyribitolphosphate-based wall teichoic acid (WTA) that is covalently anchored to the pepti...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/mmi.14472

    authors: Sumrall ET,Keller AP,Shen Y,Loessner MJ

    更新日期:2020-03-01 00:00:00

  • Bre1 and Ubp8 regulate H2B mono-ubiquitination and the reversible yeast-hyphae transition in Candida albicans.

    abstract::The reversible yeast-hyphae transition of the human fungal pathogen Candida albicans is tightly linked to its pathogenicity. In this study, we show that histone H2B mono-ubiquitination (H2Bub) at lysine 123 was maintained at a low level in the yeast state, whereas it increased significantly during yeast-to-hyphae tran...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14619

    authors: Zhu W,Fan X,Zhao Q,Xu Y,Wang X,Chen J

    更新日期:2020-10-03 00:00:00

  • The telomere system of the Streptomyces linear plasmid SCP1 represents a novel class.

    abstract::Linear plasmids and chromosomes of Streptomyces carry terminal proteins (TPs) covalently attached to the 5' ends of the DNA. Most known telomeres are conserved in primary sequence and in the potential secondary structures formed during replication. The TP that caps these telomeres is also highly conserved and its codi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05616.x

    authors: Huang CH,Tsai HH,Tsay YG,Chien YN,Wang SL,Cheng MY,Ke CH,Chen CW

    更新日期:2007-03-01 00:00:00

  • Targeting of the chemotaxis methylesterase/deamidase CheB to the polar receptor-kinase cluster in an Escherichia coli cell.

    abstract::Chemotactic adaptation to persisting stimulation involves reversible methylation of the chemoreceptors that form complexes with the histidine kinase CheA at a cell pole. The methyltransferase CheR targets to the C-terminal NWETF sequence of the chemoreceptor. In contrast, localization of the methylesterase CheB is lar...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04176.x

    authors: Banno S,Shiomi D,Homma M,Kawagishi I

    更新日期:2004-08-01 00:00:00

  • The coupling between ftsZ transcription and initiation of DNA replication is not mediated by the DnaA-boxes upstream of ftsZ or by DnaA.

    abstract::The DnaA protein of Escherichia coli is a multi-functional protein which, In addition to promoting initiation of replication, can regulate the initiation or termination of transcription of a variety of genes. It acts by binding to DNA at a defined sequence, termed a DnaA-box. Three candidate DnaA-boxes which occur wit...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1996.6431365.x

    authors: Smith RW,McAteer S,Masters M

    更新日期:1996-07-01 00:00:00

  • A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid.

    abstract::The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is charact...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14614

    authors: Kamal SM,Cimdins-Ahne A,Lee C,Li F,Martín-Rodríguez AJ,Seferbekova Z,Afasizhev R,Wami HT,Katikaridis P,Meins L,Lünsdorf H,Dobrindt U,Mogk A,Römling U

    更新日期:2020-09-28 00:00:00

  • Proteome analysis of Escherichia coli K-12 by two-dimensional native-state chromatography and MALDI-MS.

    abstract::To identify proteins expressed in Escherichia coli K-12 MG1655 during exponential growth in defined medium, we separated soluble proteins of E. coli over two dimensions of native-state high-performance liquid chromatography, and examined the components of the protein mixtures in each of 380 fractions by peptide mass f...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03294.x

    authors: Champion MM,Campbell CS,Siegele DA,Russell DH,Hu JC

    更新日期:2003-01-01 00:00:00

  • Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel.

    abstract::Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04290.x

    authors: Vimberg V,Xiong L,Bailey M,Tenson T,Mankin A

    更新日期:2004-10-01 00:00:00

  • pH-regulated activation and release of a bacteria-associated phospholipase C during intracellular infection by Listeria monocytogenes.

    abstract::Listeria monocytogenes grows in the cytosol of mammalian cells and spreads from cell to cell without exiting the intracellular milieu. During cell-cell spread, bacteria become transiently entrapped in double-membrane vacuoles. Escape from these vacuoles is mediated in part by a bacterial phospholipase C (PC-PLC), whos...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2000.01708.x

    authors: Marquis H,Hager EJ

    更新日期:2000-01-01 00:00:00

  • Reversal of an epigenetic switch governing cell chaining in Bacillus subtilis by protein instability.

    abstract::Bacillus subtilis forms long chains of cells during growth and biofilm formation. Cell separation is mediated by autolysins, whose genes are under the negative control of a heteromeric complex composed of the proteins SinR and SlrR. Formation of the SinR-SlrR complex is governed by a self-reinforcing, double-negative ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2010.07335.x

    authors: Chai Y,Kolter R,Losick R

    更新日期:2010-10-01 00:00:00

  • Mitochondrial beta-oxidation in Aspergillus nidulans.

    abstract::Beta-oxidation (beta-ox) occurs exclusively in the peroxisomes of Saccharomyces cerevisiae and other yeasts, leading to the supposition that fungi lack mitochondrial beta-ox. Here we present unequivocal evidence that the filamentous fungus Aspergillus nidulans houses both peroxisomal and mitochondrial beta-ox. While g...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04340.x

    authors: Maggio-Hall LA,Keller NP

    更新日期:2004-12-01 00:00:00

  • Thermostabilization of cellulosomal endoglucanase EngB from Clostridium cellulovorans by in vitro DNA recombination with non-cellulosomal endoglucanase EngD.

    abstract::Enhancement of enzyme thermostability by protein engineering gives us information about the thermostabilization mechanism as well as advantages for industrial use of enzymes. In this study, we enhanced the thermostability of endoglucanase EngB, one component of the cellulase complex (cellulosome) from Clostridium cell...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2002.03049.x

    authors: Murashima K,Kosugi A,Doi RH

    更新日期:2002-08-01 00:00:00

  • Efficient repression by a heterodimeric repressor in Escherichia coli.

    abstract::Previous studies with purified variants of the 434 repressor having different operator-binding specificities have demonstrated the interactions of a heterodimeric repressor with a hybrid operator site. The present study investigates the interactions between the 434 repressor and its operator site. The optimum 434 oper...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1992.tb01480.x

    authors: Webster C,Merryweather A,Brammar W

    更新日期:1992-02-01 00:00:00

  • Cell-cell communication in filamentous cyanobacteria.

    abstract::Although cytoplasmic bridges between adjacent cells in the filaments of nitrogen-fixing cyanobacteria have been known for decades, the existence also of a continuous periplasm along the filaments raised the possibility that alternative modes of communication between cells could be utilized. The latter hypothesis was i...

    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章

    doi:10.1111/j.1365-2958.2008.06475.x

    authors: Haselkorn R

    更新日期:2008-11-01 00:00:00

  • Developmental regulation of the glyoxylate cycle in the human pathogen Penicillium marneffei.

    abstract::Penicillium marneffei is a thermally dimorphic opportunistic human pathogen with a saprophytic filamentous hyphal form at 25 degrees C and a pathogenic unicellular yeast form at 37 degrees C. During infection. P. marneffei yeast cells exist intracellularly in macrophages. To cope with nutrient deprivation during the i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05477.x

    authors: Cánovas D,Andrianopoulos A

    更新日期:2006-12-01 00:00:00

  • An SOS-inducible defective retronphage (phi R86) in Escherichia coli strain B.

    abstract::In Escherichia coli, RecA protein regulates the DNA damage-inducible survival-enhancing SOS response. Mutant allele recA730, which causes constitutive SOS expression, is lethal at high temperatures in B/r, a derivative of wild-type B, but not in K-12 or in certain B/r--K-12 hybrids. We present evidence that killing is...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1992.tb01461.x

    authors: Kirchner J,Lim D,Witkin EM,Garvey N,Roegner-Maniscalco V

    更新日期:1992-10-01 00:00:00

  • cGMP homeostasis in malaria parasites-The key to perceiving and integrating environmental changes during transmission to the mosquito.

    abstract::Malaria-causing parasites are transmitted from humans to mosquitoes when developmentally arrested gametocytes are taken up by a female Anopheles during a blood meal. The changes in environment from human to mosquito activate gametogenesis, including a drop in temperature, a rise in pH, and a mosquito-derived molecule,...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/mmi.14633

    authors: Brochet M,Balestra AC,Brusini L

    更新日期:2020-10-28 00:00:00

  • Mechanism of polysialic acid chain elongation in Escherichia coli K1.

    abstract::Understanding the mechanisms of polysialic acid synthesis in Escherichia coli K1 requires a molecular description of the polymerase complex. Since the number of potential models explaining polysialic acid assembly would be constrained if only one sialyltransferase were required for this process, the phenotypes of a si...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1990.tb00629.x

    authors: Steenbergen SM,Vimr ER

    更新日期:1990-04-01 00:00:00

  • The ins and outs of bacterial iron metabolism.

    abstract::Iron is a critical nutrient for the growth and survival of most bacterial species. Accordingly, much attention has been paid to the mechanisms by which host organisms sequester iron from invading bacteria and how bacteria acquire iron from their environment. However, under oxidative stress conditions such as those enc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/mmi.12709

    authors: Frawley ER,Fang FC

    更新日期:2014-08-01 00:00:00

  • FtsA reshapes membrane architecture and remodels the Z-ring in Escherichia coli.

    abstract::Cell division in prokaryotes initiates with assembly of the Z-ring at midcell, which, in Escherichia coli, is tethered to the inner leaflet of the cytoplasmic membrane through a direct interaction with FtsA, a widely conserved actin homolog. The Z-ring is comprised of polymers of tubulin-like FtsZ and has been suggest...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13902

    authors: Conti J,Viola MG,Camberg JL

    更新日期:2018-02-01 00:00:00

  • Co-operative autoregulation of a replication protein gene.

    abstract::In this work we present the localization and characterization of the repl promoter (Prepl) and show aspects of the regulation. Comparison of Prepl with other autoregulated replication protein gene promoters revealed similarities, but Prepl differs from some of these characterized promoters in not being regulated by th...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1991.tb01861.x

    authors: Gammie AE,Crosa JH

    更新日期:1991-12-01 00:00:00

  • Heme catabolism in the causative agent of anthrax.

    abstract::A challenge common to all bacterial pathogens is to acquire nutrients from hostile host environments. Iron is an important cofactor required for essential cellular processes such as DNA repair, energy production and redox balance. Within a mammalian host, most iron is sequestered within heme, which in turn is predomin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14270

    authors: Clark J,Terwilliger A,Nguyen C,Green S,Nobles C,Maresso A

    更新日期:2019-08-01 00:00:00

  • Decorin-binding adhesins from Borrelia burgdorferi.

    abstract::Lyme disease is a tick-transmitted infection caused by the spirochete Borrelia burgdorferi. Ticks deposit B. burgdorferi into the dermis of the host, where they eventually become associated with collagen fibres. We demonstrated previously that B. burgdorferi is unable to bind collagen, but can bind the collagen-associ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1998.01103.x

    authors: Guo BP,Brown EL,Dorward DW,Rosenberg LC,Höök M

    更新日期:1998-11-01 00:00:00

  • Mismatch repair and the regulation of phase variation in Neisseria meningitidis.

    abstract::Neisseria meningitidis controls the expression of several genes involved in host adaptation by a process known as phase variation. The phase variation frequency of haemoglobin (Hb) receptors among clinical isolates of serogroups A, B and C differed drastically, ranging from approximately 10(-6) to 10(-2) cfu-1. Freque...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02408.x

    authors: Richardson AR,Stojiljkovic I

    更新日期:2001-05-01 00:00:00

  • An unusual pattern of protein expression and localization of yeast alanyl-tRNA synthetase isoforms.

    abstract::Previous studies have shown that in Saccharomyces cerevisiae the mitochondrial and cytoplasmic forms of alanyl-tRNA synthetase are encoded by a single nuclear gene, ALA1, through alternative use of in-frame successive ACG triplets and a downstream AUG triplet. Here we show that despite the obvious participation of the...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05083.x

    authors: Huang HY,Tang HL,Chao HY,Yeh LS,Wang CC

    更新日期:2006-04-01 00:00:00

  • Identification of a virulence gene cluster of Mycobacterium tuberculosis by signature-tagged transposon mutagenesis.

    abstract::Tuberculosis remains the greatest cause of death worldwide due to a single pathogen. In order to identify the genes required for the pathogenicity of Mycobacterium tuberculosis, a functional genomic approach was developed. A library of signature-tagged transposon mutants of this bacterium was constructed and screened ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1999.01593.x

    authors: Camacho LR,Ensergueix D,Perez E,Gicquel B,Guilhot C

    更新日期:1999-10-01 00:00:00