TLC of some free amino acids from sanguine plasma.

abstract：:In terms of risk assessment especially for the impurities with different ultraviolet (UV) absorptions in 16-membered macrolides produced by fermentation, quantification without the availability of corresponding reference substances currently poses a challenge. In this study, a reliable method was established for the a...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Wang J,Liu G,Zhu B,Tang L

更新日期：2020-05-30 00:00:00

abstract：:This paper deals with the chiral separation of optical isomers of beta-methyl-amino acids by CE and HPLC using the principle of ligand-exchange. Capillary zone electrophoresis was carried out using Cu(II) complexes of L-4-hydroxyproline (L-4-Hypro), N-(2-hydroxypropyl)-L-4-hydroxyproline (HP-L-4-Hypro) and N-(2-hydrox...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Grobuschek N,Schmid MG,Tuscher C,Ivanova M,Gübitz G

更新日期：2002-01-15 00:00:00

abstract：:6-O-Succinil-beta-cyclodextrin (CDsuc6) was synthesized with very good yield by one pot synthesis and characterized by NMR spectroscopy and ESI-MS. It was used as a chiral selector in capillary electrophoresis to resolve catecholamine racemates, namely norepinephrine, epinephrine, terbutaline and norphenilephrine. The...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cucinotta V,Giuffrida A,Maccarrone G,Messina M,Puglisi A,Torrisi A,Vecchio G

更新日期：2005-04-29 00:00:00

abstract：:A rapid and simple LC-MS/MS method was developed and validated for the simultaneous determination of coniferyl ferulate (CF) and its metabolite coniferyl alcohol (CA) using bavachromene as an internal standard (IS). A TSQ Quantum Access mass spectrometer was operated under selected-reaction monitoring mode using negat...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Dai X,Pang L,Zhang Z,Yang C,Li Y

更新日期：2017-11-30 00:00:00

abstract：:A specific and reliable liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed for the simultaneous determination of tetrahydropalmatine (THP), tetrahydroberineper (THB), tetrahydrocoptisine (THC) and corydaline (CDL) in rat plasma using jatrorrhizine as internal standard. Plasma s...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Wang S,Liu L,Wang L,Jiang P,Xiang L,Zhang W,Liu R

更新日期：2013-01-01 00:00:00

abstract：:CpG oligodeoxynucleotide 107 (CpG ODN107) could be used as a novel radiosensitizer for glioma. Herein, a novel and sensitive reversed-phase HPLC coupled with electrospray triple quadrupole mass spectrometry (LC-MS/MS) following a one-step C18 solid-phase extraction (SPE) for biological matrix removal was developed and...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cen Y,Li X,Liu D,Pan F,Cai Y,Li B,Peng W,Wu C,Jiang W,Zhou H

更新日期：2012-11-01 00:00:00

abstract：:Statistical modelling and experimental design (SMED) are essential tools for the development and understanding of complicated products and processes. SMED allows efficient experimentation in which all or a large subset of factors are together varied over a set of experiments, in contrast to the traditional approach of...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kettaneh-Wold N

更新日期：1991-01-01 00:00:00

abstract：:The supposed 5-LO inhibitory activity of two N-omega-ethoxycarbonyl-4-quinolones was tested determining leukotriene B4 (LTB4) in RBL-1 cell cultures, pretreated with the two compounds of interest. LTB4, obtained by solid-phase extraction (SPE) from cell cultures supernatants, was determined by micellar electrokinetic ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Bossù E,Aglianò AM,Desideri N,Sestili I,Porrà R,Grandilone M,Quaglia MG

更新日期：1999-03-01 00:00:00

abstract：:A high-performance liquid chromatographic method for the simultaneous determination of 5-aminosalicylic acid (5-ASA), acetyl-5-aminosalicylic acid (Ac-5-ASA) and 2,5-dihydroxybenzoic acid (5-HSA) in human endoscopic intestinal biopsy with electrochemical detection has been developed and validated. A liquid-liquid extr...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Palumbo G,Carlucci G,Mazzeo P,Frieri G,Pimpo MT,Fanini D

更新日期：1995-12-01 00:00:00

abstract：:The chromatographic behaviour (retention, selectivity, peak shape and resolution) of seven tricyclic antidepressants (TCAs), amitryptiline, clomipramine, doxepin, imipramine, maprotiline, nortryptiline and trimipramine, was examined. Conventional unendcapped Cs and C18 columns and an endcapped XTerra MS C18 column rec...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ruiz-Angel MJ,Carda-Broch S,Simó-Alfonso EF,García-Alvarez-Coque MC

更新日期：2003-04-24 00:00:00

abstract：:Second derivative UV spectrophotometry proved to be a useful tool for the determination of acetaminophen, even in the presence of high amounts of the impurity, 4-aminophenol and the background effect caused by dissolved excipients of some dosage forms. The fluorimetric method used for the estimation of 4-aminophenol i...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Milch G,Szabó E

更新日期：1991-01-01 00:00:00

abstract：:Quantitative IR- and Raman spectroscopic determinations of four cephalosporin antibiotics in six solid binary mixtures have been conducted. This is a new approach for spectroscopic determination of these antibiotics, since the corresponding quantitative analysis in solution only has been reported so far. The correlati...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Koleva BB,Kolev TM,Spiteller M

更新日期：2008-09-10 00:00:00

abstract：:Spectroscopic and chromatographic methods (HPLC, HPLC-MS, NMR) were used to observe, separate and identify products of radiolysis of thiamphenicol (TF), irradiated in the solid state at room temperature and atmospheric pressure with an electron beam from a linear accelerator to doses between 25 and 800 kGy. Nine produ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Marciniec B,Stawny M,Danikiewicz W,Spólnik G,Jaroszkiewicz E,Needham M

更新日期：2010-12-01 00:00:00

abstract：:An extracellular thermostable antibacterial peptide designated as MFAP9 was purified from marine Aspergillus fumigatus BTMF9 by ammonium sulfate precipitation followed by ion exchange chromatography on a DEAE-sepharose column. The molecular weight of MFAP9 was found to be∼3 kDa in SDS-PAGE gel corresponding a single i...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kollakalnaduvil Raghavan RM,Ali Pannippara M,Kesav S,Mathew A,G Bhat S,Hatha Aa M,Kk E

更新日期：2021-02-05 00:00:00

abstract：:Thermodegradation of labile compounds in the hot injection port of a gas chromatograph causes many problems and can lead to ambiguous, poorly reproducible results. Splitless injection used in trace analysis in order to achieve a lower detection limit may even enhance the difficulties due to the longer residence time o...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Klick S

更新日期：1995-04-01 00:00:00

abstract：:The interactions between gemcitabine hydrochloride (GEM) and bovine serum albumin (BSA) or human serum albumin (HSA) have been studied by spectroscopic techniques. By the analysis of fluorescence spectrum and fluorescence intensity, it was observed that the GEM has a strong ability to quench the intrinsic fluorescence...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kandagal PB,Ashoka S,Seetharamappa J,Shaikh SM,Jadegoud Y,Ijare OB

更新日期：2006-05-03 00:00:00

abstract：:A reversed-phase ion pair liquid chromatographic method (RP-LC) for the determination of dehydroascorbic acid (DHA) and ascorbic acid (AA) and also acetaminophen, which is combined in pharmaceuticals, is proposed and validated. AA and acetaminophen were analyzed directly, while DHA was determined after pre-column deri...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gioia MG,Andreatta P,Boschetti S,Gatti R

更新日期：2008-09-29 00:00:00

abstract：:An analytical method that allows simultaneous analysis of some (semi-)volatile drugs and additives in pharmaceuticals for topical use was developed using classical static headspace as sampling technique combined with gas chromatography (HS-GC). The capillary column used, RSL-200, showed good selectivity towards all th...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Sitaramaraju Y,van Hul A,Wolfs K,Van Schepdael A,Hoogmartens J,Adams E

更新日期：2008-08-05 00:00:00

abstract：:Four microemulsion liquid chromatography (MELC) systems and one micellar liquid chromatography (MLC) system have been evaluated as potential high-throughput screening platforms capable of modeling the partitioning behaviors of drug compounds in an n-octanol/water system and predicting their lipophilicity (i.e., log P ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Xu L,Li L,Huang J,Yu S,Wang J,Li N

更新日期：2015-01-01 00:00:00

abstract：:The increasing complexity of novel biotherapeutics such as bispecific antibodies or fusion proteins raises new challenges for functional characterization. When compared to standard antibodies, two individual interactions and the inter-dependency of binding events need to be considered for bispecific antibodies. We hav...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Meschendoerfer W,Gassner C,Lipsmeier F,Regula JT,Moelleken J

更新日期：2017-01-05 00:00:00

abstract：:This study addresses the lack of data on the stability of carfilzomib, a newly approved proteasome-inhibiting anticancer drug. A new stability-indicating UHPLC-UV method for analysis of carfilzomib was developed and validated within the concentrations of 10-250 μg/mL. The aforementioned method was utilized to evaluate...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Sestak V,Roh J,Klepalova L,Kovarikova P

更新日期：2016-05-30 00:00:00

abstract：:Bepridil is potent inhibitor of Na+, K+ and Ca+ channel in cardiomyocytes. It has demonstrated strong antianginal effect with type I antiarrhythmic and with minimum antihypertensive therapeutic effect. Till date, a specific LC-MS/MS method to quantify Bepridil concentrations in biological matrix have not been reported...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Patel BD,Palakundam N,Alamanda R,Gadekar AR,Raje AA,Ameta R

更新日期：2019-08-05 00:00:00

abstract：:Lipid nanoparticles, especially liposomes and lipid/nucleic acid complexed nanoparticles have shown great success in the pharmaceutical industry. Their success is attributed to stable drug loading, extended pharmacokinetics, reduced off-target side effects, and enhanced delivery efficiency to disease targets with form...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Fan Y,Marioli M,Zhang K

更新日期：2021-01-05 00:00:00

abstract：:A simple and sensitive method is proposed for the determination of boldine by first-derivative synchronous spectrofluorimetry based on its native fluorescence in 0.1 N sulphuric acid. The constant wavelength difference (delta lambda = lambda em--lambda ex) chosen to optimize the determination is 90 nm. The linear conc...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Vilchez JL,Sánchez-Palencia G,Avidad R,Navalón A

更新日期：1994-03-01 00:00:00

abstract：:Pharmaceutical excipients containing volatile odor-active molecules can be used in pharmaceutical development to increase patients' compliance. However, capturing the molecular composition of these odor-active substances is challenging. Therefore, guidance for the analytical investigation of these excipients should be...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kiene FE,Pein M,Thommes M

更新日期：2015-06-10 00:00:00

abstract：:The development of high performance liquid chromatography method on amylose-based stationary phases (Chiralpak AD) has permitted to achieve the preparative enantioseparation of one benzimidazole derivative, potent-AMP-kinase (AMPK) activator with satisfactory yields. Analytical enantioseparation method was optimized a...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Vaccher MP,Charton J,Guelzim A,Caignard DH,Bonte JP,Vaccher C

更新日期：2008-04-14 00:00:00

abstract：:A selective and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed for the simultaneous determination of Euphorbia factor L(1), Euphorbia factor L(2) and Euphorbia factor L(3) from Euphorbiae semen in rat plasma. Larotaxel was added to a 200 μL plasma sample as the internal standard (IS). T...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Meng X,Zhao X,Long Z,Yuan Y,Zhuang H,Bi K,Chen X

更新日期：2013-01-01 00:00:00

abstract：:Capillary zone electrophoresis is a technique that is rapidly gaining popularity in the pharmaceutical industry because of the wide range of compounds applicable to the technique. These range from small biologically active molecules through to large nucleotides and proteins. This review outlines the historical develop...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Smith NW,Evans MB

更新日期：1994-05-01 00:00:00

abstract：:Antibody charge heterogeneity is one of the major product-related variants in recombinant biopharmaceuticals, which has been commonly monitored by imaged capillary isoelectric focusing (icIEF). Due to the challenges with sample recovery and fractionation, other charge-based analytical approaches have been explored as ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Liu S,Madren S,Feng P,Sosic Z

更新日期：2020-06-05 00:00:00

abstract：:The classification of an impurity of a drug substance as genotoxic means that the "threshold of toxicological concern" (TTC) value of 1.5 μg/day intake, considered to be associated with an acceptable risk, should be the admissible limit in the raw material and that leads to new analytical challenges. In this study, re...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： García A,Rupérez FJ,Ceppa F,Pellati F,Barbas C

更新日期：2012-03-05 00:00:00