Abstract:
:A selective and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed for the simultaneous determination of Euphorbia factor L(1), Euphorbia factor L(2) and Euphorbia factor L(3) from Euphorbiae semen in rat plasma. Larotaxel was added to a 200 μL plasma sample as the internal standard (IS). The plasma sample was extracted by 2 mL ether and separated on an Elite C(18) column (150 mm × 4.6 mm, 5 μm) with the mobile phase of methanol-water performing gradient elution within 11.0 min. All the three analytes were detected in the selected ion monitoring (SIM) mode with positive electrospray ionization. The calibration curves were linear in the range of 2.0-200 ng/mL and the lower limits of quantification (LLOQ) were 2.0 ng/mL for all the three analytes. The intra-day and inter-day precisions were all within 8.6% and 14.6% for the three analytes, while the accuracy was less than 9.7% and 7.5%, respectively. The validated method was firstly and successfully applied to the pharmacokinetic study of Euphorbia factor L(1), Euphorbia factor L(2) and Euphorbia factor L(3) from Euphorbiae semen after oral administration in rat plasma.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Meng X,Zhao X,Long Z,Yuan Y,Zhuang H,Bi K,Chen Xdoi
10.1016/j.jpba.2012.09.005subject
Has Abstractpub_date
2013-01-01 00:00:00pages
299-305eissn
0731-7085issn
1873-264Xpii
S0731-7085(12)00494-3journal_volume
72pub_type
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