Abstract:
:An electrochemical DNA nano-biosensor is prepared by immobilization of double stranded DNA (dsDNA) onto a mixed self-assembled monolayer (SAM) composed of azide- and hydroxyl-terminated thiols. The SAMs- and dsDNA-modified gold electrodes were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The interaction of spermidine with DNA was studied by differential pulse voltammetry at the DNA-modified electrode. The decrease in the guanine oxidation peak current was used to study the interaction. The binding constant (K), obtained by differential pulse voltammetry, was 1.85 x 10(5)M(-1). A linear dependence of the guanine peak currents was observed in the range of 1.6-70.4 microM spermidine, with a detection limit of 0.72 microM and r=0.994 by using differential pulse voltammetry.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Mehdinia A,Kazemi SH,Bathaie SZ,Alizadeh A,Shamsipur M,Mousavi MFdoi
10.1016/j.jpba.2008.12.035subject
Has Abstractpub_date
2009-04-05 00:00:00pages
587-93issue
3eissn
0731-7085issn
1873-264Xpii
S0731-7085(08)00697-3journal_volume
49pub_type
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