Abstract：
:Sinoporphyrin sodium (DVDMS) is a new photosensitizer (PS) and it is used in photodynamic therapy (PDT) against tumor. In this paper, a simple, rapid and specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the quantitation of DVDMS in human plasma was developed. The analytes were extracted from plasma samples using liquid-liquid extraction (LLE) after addition of testosterone (internal standard) and chromatographed on an AQUITY UPLC Protein BEH C4 column (50 × 2.1 mm, i.d. 1.7 μm) thermostatted at 40 °C with acetonitrile-water (0.1% formic acid and 0.1 mM K2EDTA) as the gradient mobile phase at flow rate of 0.5 mL/min. The detection was performed on an API 5500 mass spectrometer coupled with electrospray ionization (ESI) source in positive mode. The multiple reactions monitoring (MRM) transitions of m/z 1143.6→563.2 and m/z 289.3→109.1 were used to quantify DVDMS and IS, respectively. The assay was validated over the concentration range of 30-3000 ng/mL. Precision and accuracy are in accordance with the generally accepted criteria for bioanalytical methods. The extraction recovery and the matrix effect were investigated. This method was successfully applied to pharmacokinetic (PK) study of DVDMS in Chinese patients with solid tumor after treated with DVDMS-PDT for the first time.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Zhou J,Yang F,Yuan JJ,Bo YH,Gong JF,Shen Ldoi
10.1016/j.jpba.2020.113852subject
Has Abstractpub_date
2021-02-20 00:00:00pages
113852eissn
0731-7085issn
1873-264Xpii
S0731-7085(20)31739-8journal_volume
195pub_type
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