Abstract：
:An account is given of the development of tests for the bio-assay of insulin and the progress made, at the Wellcome Foundation Limited, during the last 30 years in reducing the number of animals used in the testing of insulin for the treatment of Diabetes Mellitus. The progressive evolution of methods shows how the number of mice used per sample has already been reduced five-fold and an ongoing development of in vitro methods, particularly high-performance liquid chromatography (HPLC), has enabled Wellcome to recommend to the regulatory authorities alternative procedures to replace animal testing.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Trethewey Jdoi
10.1016/0731-7085(89)80083-4subject
Has Abstractpub_date
1989-01-01 00:00:00pages
189-97issue
2eissn
0731-7085issn
1873-264Xpii
0731-7085(89)80083-4journal_volume
7pub_type
杂志文章,评审abstract：:Psoralea corylifolia L. (Fabaceae) is a traditional Chinese medicine with many beneficial effects in medical therapies. Bakuchiol was the main active ingredient of Psoralea corylifolia L. In this study, a novel method of pre-column derivatization with dansyl chloride followed by analysis of ultra high-performance liqu...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2012.11.001
更新日期：2013-03-05 00:00:00
abstract：:Solid phase extraction applied to plant matrices is nowadays a well validated technique allowing the concentration and purification of selected secondary metabolites for subsequent analysis. In this short communication we screened the efficiency of 16 selected solid supports including layered structures (hydrotalcites...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2020.113348
更新日期：2020-08-05 00:00:00
abstract：:Acute myeloid leukemia (AML) is a malignant disease originating from bone marrow hematopoietic stem cells, characterized by anemia, hemorrhage, fever, and infection, with low survival rate. However, the pathogenesis of AML is not fully understood at present. In this work, an integrated approach based untargeted metabo...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2020.113824
更新日期：2021-02-20 00:00:00
abstract：:A micellar electrokinetic chromatographic (MEKC) method was developed for the quantification of lovastatin and simvastatin, cholesterol lowering agents in pharmaceutical dosage forms. Lovastatin and simvastatin were separated using an electrolyte system consisting of 12% acetonitrile (v/v) in 25 mM sodium borate buffe...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(02)00128-0
更新日期：2002-07-20 00:00:00
abstract：:Optimization of extraction and chromatographic conditions is essential for the simultaneous analysis of drugs having different physico-chemical properties, especially for in vivo applications. The present work describes concurrent estimation of metformin (MET) and three sodium-glucose co-transporter 2 (SGLT-2) inhibit...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2019.112790
更新日期：2019-10-25 00:00:00
abstract：:Hair analysis is a useful tool for establishing long-term drug intake. Segmental analysis, in particular, where the hair is cut into defined segments, can potentially provide a calendar of patients' drug intake as drugs are incorporated into the growing hair through the bloodstream with an average growth rate of 1 cm ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2020.113510
更新日期：2020-10-25 00:00:00
abstract：:A reversed-phase high-performance liquid chromatography (HPLC) method with dual optical rotation/UV absorbance detection has been developed for the determination of enantiomeric purity of ephedrine hydrochloride and pseudoephedrine hydrochloride using an achiral column. The method gave a correlation coefficient of 0.9...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/0731-7085(90)80050-y
更新日期：1990-01-01 00:00:00
abstract：:The similarities and differences of essential oil components in Pericarpium Citri Reticulatae Viride (PCRV) and Pericarpium Citri Reticulatae (PCR) were investigated by GC-MS combined with a chemometric method, named alternative moving window factor analysis (AMWFA). Furthermore, temperature-programmed retention indic...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2007.08.030
更新日期：2008-01-07 00:00:00
abstract：:Non-covalent interaction of hemicyanine dyes, derivatives of 2,3,3-trimethyl-3H-indolium with bovine serum albumin has been studied by spectral method. For this purpose, three hemicyanine dyes containing N-(2-carboxyethyl)-2,3,3-trimethyl-3H-indolium moiety were synthesized and their UV/Vis and fluorescence spectra, a...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2015.06.023
更新日期：2015-10-10 00:00:00
abstract：:Curcumin, a principal curcuminoid of turmeric, gained a lot of attention recently due to its wide spectrum of pharmacological activities in prevention and treatment of various human conditions. The current clinical study is focused on the determination of efficacy and tolerability of curcumin and vitamin D3 combinatio...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2018.04.034
更新日期：2018-07-15 00:00:00
abstract：:A gas chromatographic method to measure urinary levels of the central nervous system stimulant fencamfamine and some of its metabolites is described. When 100 mg fencamfamine was given orally to four horses the parent drug could not be detected in the urine. After enzymatic hydrolysis of the urine the major human meta...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/0731-7085(92)80093-3
更新日期：1992-09-01 00:00:00
abstract：:An automated system has been developed and validated for the analysis of liquid formulations (solution and suspension). The system comprises a multi-place magnetic stirrer, a diluter and a diode array UV spectrophotometer. The system can handle a wide range of drug formulation concentrations (> 200 mg ml-1) and gives ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(96)01882-1
更新日期：1997-02-01 00:00:00
abstract：:A sensitive enantioselective liquid chromatography-mass spectrometry (LC-MS) assay using a manual solid-phase extraction (SPE) procedure, a non-deuterated internal standard and an ion trap LC-MS was developed to measure (R)- and (S)-albuterol in plasma. Sample extraction from plasma was achieved by a manual SPE extrac...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(02)00734-3
更新日期：2003-04-10 00:00:00
abstract：:A reversed-phase high-performance liquid chromatographic (RP-HPLC) separation method with UV spectrophotometric detection has been developed for the determination of major components in leaves and caulomas of Schisandra chinensis Baill. The flavonols (quercetin and kaempferol) and (E)-cinnamic acid were analysed after...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(00)00539-2
更新日期：2001-03-01 00:00:00
abstract：:A simple and rapid spectrophotometric method with flow injection is proposed for determination of diphenhydramine hydrochloride in some single tertiary alkylamine pharmaceutical preparations (capsule and syrup). It is based on ion pair formation with bromocresol green in a pH 3 buffer which a yellow ion pair compound ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(02)00197-8
更新日期：2002-08-22 00:00:00
abstract：:Amentoflavone, a kind of biflavonoid existing in several medicinal plants such as Selaginella moellendorfi and Gingko biloba, possesses anti-inflammatory, antioxidant, anti-virus, anti-tumor activities. In the present study, a new reliable and sensitive UHPLC-ESI-MS/MS method was developed to determine the permeabilit...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2020.113441
更新日期：2020-09-10 00:00:00
abstract：:A procedure based on liquid chromatography-mass spectrometry (LC-MS) is described for determination of caffeine, theobromine, theophylline, taurine in different dietary supplements. After addition of tryptophan as internal standard, both solid and liquid specimens were extracted with 4 ml of hexane/isopropanol (9:1). ...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2004.11.013
更新日期：2005-03-09 00:00:00
abstract：:A health supplement used for sexual performance enhancement was sent to Health Sciences Authority of Singapore for testing. An unknown compound was detected and isolated and its structure was elucidated using NMR, high-resolution MS, ESI-MS/MS, UV and IR. The compound, dapoxetine, is reported to be a selective seroton...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2009.06.005
更新日期：2009-12-05 00:00:00
abstract：:A sensitive and specific ultra-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for the determination of nicousamide in human plasma. This method has also been validated in terms of selectivity, linearity, lower limit of quantification (LLOQ), accuracy, precision, stabili...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2013.01.025
更新日期：2013-04-15 00:00:00
abstract：:The analysis of gentamicin by liquid chromatography using a column packed with poly(styrene-divinylbenzene) and pulsed electrochemical detection on a gold electrode is described. The mobile phase consists of an aqueous solution containing sodium sulfate, tetrahydrofuran, sodium 1-octanesulfonate and a phosphate buffer...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(98)00214-3
更新日期：1998-12-01 00:00:00
abstract：:Alzheimer's disease (AD) is a neurodegenerative disease that seriously affects daily life. Schisandra chinensis (Turcz.) Baill. Fructus (SCF) and Alpinia oxyphylla Miq. Fructus (AOF) have been regarded as classical herbs for dementia since ancient times. Alpinia oxyphylla Miq.-Schisandra chinensis (Turcz.) Baill. herb...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2019.112874
更新日期：2020-01-05 00:00:00
abstract：:A chiral liquid chromatographic method was developed for the enantiomeric resolution of Pramipexole dihydrochloride monohydrate, (S)-2-amino-4,5,6,7-tetra-hydro-6-(propylamino) benzothiazole dihydrochloride monohydrate, a dopamine agonist in bulk drugs. The enantiomers of Pramipexole dihydrochloride monohydrate were r...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2006.02.024
更新日期：2006-06-16 00:00:00
abstract：:A stable isotope dilution liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) assay to measure amiodarone, the most frequently used agent for maintaining sinus rhythm in patients with atrial fibrillation, and its major metabolite desethylamiodarone in human plasma and serum was developed....
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2009.08.004
更新日期：2010-01-05 00:00:00
abstract：:A simple isocratic reversed-phase HPLC method for the determination of gabapentin and its major degradation impurity, 3,3-pentamethylene-4-butyrolactam, was developed and validated for use in the analysis of pharmaceutical tablets and capsules. Separation was achieved on a Brownlee Spheri-5 Cyano column using an aceto...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2006.12.020
更新日期：2007-04-11 00:00:00
abstract：:An analytical method that allows simultaneous analysis of some (semi-)volatile drugs and additives in pharmaceuticals for topical use was developed using classical static headspace as sampling technique combined with gas chromatography (HS-GC). The capillary column used, RSL-200, showed good selectivity towards all th...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2008.04.006
更新日期：2008-08-05 00:00:00
abstract：:A method is described for the high performance liquid chromatographic (HPLC) determination of phenylpropanolamine (PPA) based on precolumn derivatization with 4-dimethylaminobenzaldehyde (DAB) and elution from phenomenex C-18 column with methanol-water and detection by spectrophotometry at 418 nm. Linear calibration w...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(01)00371-5
更新日期：2001-09-01 00:00:00
abstract：:Ionic liquid-based salt-induced liquid-liquid extraction was developed for the first time and applied to the extraction of four active constituents, including polydatin, resveratrol, emodin, and physcion in Polygonum cuspidatum (P. cuspidatum). In this study, ionic liquid was used as extraction solvent. The dried P. c...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2018.09.050
更新日期：2019-01-30 00:00:00
abstract：:Omega-3 fatty acids are clinically useful and the two marine omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are prevalent in fish and fish oils. Omega-3 fatty acid formulations should undergo a rigorous regulatory step in order to obtain United States Food and Drug Administration (USFDA...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/j.jpba.2017.04.002
更新日期：2017-07-15 00:00:00
abstract：:A quick and sensitive method has been developed and validated for the determination of tungsten in bulk drug substance and intermediates using either Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) or Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Sample preparation is by direct dissolution w...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/s0731-7085(98)00259-3
更新日期：1999-05-01 00:00:00
abstract：:From the reaction between l-lysine and formaldehyde, Nepsilon-formyl-L-lysine was isolated by means of ion-exchange column chromatography. The identification of Nepsilon-formyl-L-lysine was carried out by ion-exchange overpressured-layer chromatography (OPLC) and 1H NMR and 13C NMR spectroscopies. The m.p. and mixed m...
journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章
doi：10.1016/0731-7085(85)80042-x
更新日期：1985-01-01 00:00:00
