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Fast analysis of glycosaminoglycans by microchip electrophoresis with in situ fluorescent detection using ethidium bromide.

Abstract：

:We analyzed some glycosaminoglycans and natural and artificial acidic polysaccharides using microchip electrophoresis in the buffer containing ethidium bromide, and found that they were successfully separated and detected within 150 s with comparable sensitivity with that of conventional electrophoresis using cellulose acetate membrane. We applied the technique to the analysis of glycosaminoglycans in pharmaceutical preparations and also in cultured cancer cells. Rapidness and easy operation of the proposed technique are quite useful for routine analysis of glycosaminoglycans.

journal_name

J Pharm Biomed Anal

journal_title

Journal of pharmaceutical and biomedical analysis

authors

Matsuno YK,Kinoshita M,Kakehi K

doi

10.1016/j.jpba.2004.11.024

subject

Has Abstract

pub_date

2005-03-09 00:00:00

pages

429-36

issue

3

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(04)00605-3

journal_volume

37

pub_type

杂志文章

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