Abstract：
:CDRI compound 81/470 is a new broad spectrum anthelmintic agent and is being developed for veterinary use. HPLC assay method for 81/470 in cow milk was developed and validated. The sample preparation consisted of protein precipitation, followed by extraction with ether. Separation was achieved on a C18 column using acetonitrile-buffer (pH 6, 50 mM) mobile phase and the compound was quantitated using fluorescence detector. The recovery of 81/470 was above 90% and was consistent over the calibration range of 10-1000 ng ml-1. Accuracy and precision were determined by analyzing replicate samples and were found to be within acceptable limits. Five freeze-thaw cycles of spiked milk and storage of processed dry residues at -30 degrees C for 7 days did not have any detrimental effect on the stability of 81/470.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Nagaraja NV,Singh SK,Gupta RCdoi
10.1016/s0731-7085(96)01913-9subject
Has Abstractpub_date
1997-05-01 00:00:00pages
1175-80issue
8eissn
0731-7085issn
1873-264Xpii
S0731-7085(96)01913-9journal_volume
15pub_type
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journal_title：Journal of pharmaceutical and biomedical analysis
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